Endogenous expression of nNOS protein in several neuronal cell lines

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Abstract

Several neuronal cell lines were screened for endogenous expression of neuronal nitric oxide synthase (nNOS) protein using Western blot analysis. Detectable levels of the nNOS protein were evident in the SK-N-SH, SH-SY5Y, and N1E-115 neuroblastoma cell lines, as well as the NG108-15 neuroblastoma × glioma hybrid. Only trace amounts were visible in Neuro2A human neuroblastoma cells. The presence of endogenously expressed nNOS in these cells may allow for the study of the interaction between nNOS and the endogenous receptor systems expressed in the same cells.

Original languageEnglish (US)
Pages (from-to)813-817
Number of pages5
JournalLife Sciences
Volume71
Issue number7
DOIs
StatePublished - Jul 5 2002

Bibliographical note

Funding Information:
This work was supported by NIH grant NS25743.

Keywords

  • Neuroblastoma cells
  • Neuronal nitric oxide synthase
  • Western blot analysis

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