Enantioseparation of amino acids and α-hydroxy acids on ligand-exchange continuous beds by capillary electrochromatography

Patrycja Puchalska, Elfriede Pittler, Marek Trojanowicz, Gerald Gübitz, Martin G. Schmid

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

A new chiral stationary phase based on continuous bed (CB) technology using L-prolinamide as a chiral selector was prepared. Its ability for enantioseparation of amino acids and a-hydroxy acids by ligand-exchange CEC was compared with that of a CB containing L-4-hydroxyproline as chiral selector. Preparation was done by a one-step in situ copolyermerization procedure using methacrylamide as monomer, vinylsulfonic acid as charge providing agent and piperazine diacrylamide as crosslinker. L-Prolinamide showed marked enantioselective properties for the separation of amino acids and α-hydroxy acids. Furthermore, we show the possibility of simultaneous enantioseparation of amino acids and α-hydroxy acids using the CB with L-4-hydroxyproline as chiral selector.

Original languageEnglish (US)
Pages (from-to)1517-1520
Number of pages4
JournalELECTROPHORESIS
Volume31
Issue number9
DOIs
StatePublished - May 1 2010
Externally publishedYes

Keywords

  • Capillary electrochromatography
  • Chiral ligand exchange
  • L-4-hydroxyproline
  • L-prolinamide
  • Monolithic phase

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