Abstract
The binding properties of S- and R-ofloxacin to poly[d(A-T)2], poly[d(G-C)2] and poly[d(I-C)2] were studied by circular dichroism (CD) and various fluorescence techniques. The spectral properties of R-ofloxacin did not change when it was mixed with poly[d(A-T)2] and poly[d(I-C)2], indicating that R-enantiomer does not interact with these polynucleotides. On the other hand, when S-ofloxacin was mixed with any polynucleotide, or R-enantiomer with poly[d(G-C)2], characteristic changes in CD and fluorescence were observed. Therefore, it is clear that enantiomers of ofloxacin selectively recognize B-form DNA. The overall spectral properties of the ofloxacin-polynucleotide complex are similar to those of the norfloxacin-polynucleotide complex [Eur. J. Biochem. 267 (2000) 6018], suggesting that this quinolone also binds in the minor groove of DNA and therefore it may be partially inserted between DNA bases or interact with purine bases.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 197-203 |
| Number of pages | 7 |
| Journal | European Journal of Pharmaceutical Sciences |
| Volume | 18 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 2003 |
| Externally published | Yes |
Bibliographical note
Funding Information:The authors acknowledge the financial support from the Korea Science and Engineering Foundation (Grant no. R01-2000-000-00043-0).
Keywords
- DNA
- Enantioselective binding
- Ofloxacin
- Optical spectroscopy
- Quinolone