Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation

Feng Li; Narayana M. Upadhyaya; Jana Sperschneider; Oadi Matny; Hoa Nguyen-Phuc; Rohit Mago; Castle Raley; Marisa E. Miller; Kevin A.T. Silverstein; Eva Henningsen; Cory D. Hirsch; Botma Visser; Zacharias A. Pretorius; Brian J. Steffenson; Benjamin Schwessinger; Peter N. Dodds; Melania Figueroa

doi:10.1038/s41467-019-12927-7

Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation

Feng Li, Narayana M. Upadhyaya, Jana Sperschneider, Oadi Matny, Hoa Nguyen-Phuc, Rohit Mago, Castle Raley, Marisa E. Miller, Kevin A.T. Silverstein, Eva Henningsen, Cory D. Hirsch, Botma Visser, Zacharias A. Pretorius, Brian J. Steffenson, Benjamin Schwessinger, Peter N. Dodds, Melania Figueroa

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104 Scopus citations

Abstract

Parasexuality contributes to diversity and adaptive evolution of haploid (monokaryotic) fungi. However, non-sexual genetic exchange mechanisms are not defined in dikaryotic fungi (containing two distinct haploid nuclei). Newly emerged strains of the wheat stem rust pathogen, Puccinia graminis f. sp. tritici (Pgt), such as Ug99, are a major threat to global food security. Here, we provide genomics-based evidence supporting that Ug99 arose by somatic hybridisation and nuclear exchange between dikaryons. Fully haplotype-resolved genome assembly and DNA proximity analysis reveal that Ug99 shares one haploid nucleus genotype with a much older African lineage of Pgt, with no recombination or chromosome reassortment. These findings indicate that nuclear exchange between dikaryotes can generate genetic diversity and facilitate the emergence of new lineages in asexual fungal populations.

Original language	English (US)
Article number	5068
Journal	Nature communications
Volume	10
Issue number	1
DOIs	https://doi.org/10.1038/s41467-019-12927-7
State	Published - Dec 1 2019

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© 2019, Crown.

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Li, F., Upadhyaya, N. M., Sperschneider, J., Matny, O., Nguyen-Phuc, H., Mago, R., Raley, C., Miller, M. E., Silverstein, K. A. T., Henningsen, E., Hirsch, C. D., Visser, B., Pretorius, Z. A., Steffenson, B. J., Schwessinger, B., Dodds, P. N., & Figueroa, M. (2019). Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation. Nature communications, 10(1), Article 5068. https://doi.org/10.1038/s41467-019-12927-7

Li, F, Upadhyaya, NM, Sperschneider, J, Matny, O, Nguyen-Phuc, H, Mago, R, Raley, C, Miller, ME, Silverstein, KAT, Henningsen, E, Hirsch, CD, Visser, B, Pretorius, ZA, Steffenson, BJ, Schwessinger, B, Dodds, PN & Figueroa, M 2019, 'Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation', Nature communications, vol. 10, no. 1, 5068. https://doi.org/10.1038/s41467-019-12927-7

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Emergence of the Ug99 lineage of the wheat stem rust pathogen through somatic hybridisation

Abstract

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