Embryological and genetic manipulation of chick development

Laura S Gammill; Bridget Jacques-Fricke; Julaine Roffers-Agarwal

doi:10.1007/978-1-4939-9009-2_6

Embryological and genetic manipulation of chick development

Laura S Gammill, Bridget Jacques-Fricke, Julaine Roffers-Agarwal

Research output: Chapter in Book/Report/Conference proceeding › Chapter

7 Scopus citations

Abstract

The ability to combine embryological manipulations with gene function analysis in an amniote embryo makes the chick a valuable system for the vertebrate developmental biologist. This chapter describes methods for those unfamiliar with the chick system wishing to initiate experiments in their lab. After outlining methods to prepare chick embryos, protocols are provided for introducing beads or cells expressing secreted factors, and for culturing tissue explants as a means of assessing development in vitro. Approaches to achieve gain of function and loss of function (morpholino oligonucleotides) in chick are outlined, and methods for introducing these reagents by electroporation are detailed.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	75-97
Number of pages	23
Volume	1920
DOIs	https://doi.org/10.1007/978-1-4939-9009-2_6
State	Published - 2019

Publication series

Name	Methods in Molecular Biology
Volume	1920
ISSN (Print)	1064-3745

Keywords

Chick
Electroporation
Embryo culture
Morpholino oligonucleotide
Gene Transfer Techniques
Gene Expression
Signal Transduction
Embryo Culture Techniques
Chick Embryo
Gene Knockdown Techniques
Animals
Genetic Engineering
Gene Expression Regulation, Developmental
Embryonic Development/genetics
Transgenes
Genes, Reporter

PubMed: MeSH publication types

Journal Article

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10.1007/978-1-4939-9009-2_6

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title = "Embryological and genetic manipulation of chick development",

abstract = "The ability to combine embryological manipulations with gene function analysis in an amniote embryo makes the chick a valuable system for the vertebrate developmental biologist. This chapter describes methods for those unfamiliar with the chick system wishing to initiate experiments in their lab. After outlining methods to prepare chick embryos, protocols are provided for introducing beads or cells expressing secreted factors, and for culturing tissue explants as a means of assessing development in vitro. Approaches to achieve gain of function and loss of function (morpholino oligonucleotides) in chick are outlined, and methods for introducing these reagents by electroporation are detailed.",

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author = "Gammill, \{Laura S\} and Bridget Jacques-Fricke and Julaine Roffers-Agarwal",

year = "2019",

doi = "10.1007/978-1-4939-9009-2\_6",

language = "English (US)",

volume = "1920",

series = "Methods in Molecular Biology",

publisher = "Humana Press Inc.",

pages = "75--97",

booktitle = "Methods in Molecular Biology",

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T1 - Embryological and genetic manipulation of chick development

AU - Gammill, Laura S

AU - Jacques-Fricke, Bridget

AU - Roffers-Agarwal, Julaine

PY - 2019

Y1 - 2019

N2 - The ability to combine embryological manipulations with gene function analysis in an amniote embryo makes the chick a valuable system for the vertebrate developmental biologist. This chapter describes methods for those unfamiliar with the chick system wishing to initiate experiments in their lab. After outlining methods to prepare chick embryos, protocols are provided for introducing beads or cells expressing secreted factors, and for culturing tissue explants as a means of assessing development in vitro. Approaches to achieve gain of function and loss of function (morpholino oligonucleotides) in chick are outlined, and methods for introducing these reagents by electroporation are detailed.

AB - The ability to combine embryological manipulations with gene function analysis in an amniote embryo makes the chick a valuable system for the vertebrate developmental biologist. This chapter describes methods for those unfamiliar with the chick system wishing to initiate experiments in their lab. After outlining methods to prepare chick embryos, protocols are provided for introducing beads or cells expressing secreted factors, and for culturing tissue explants as a means of assessing development in vitro. Approaches to achieve gain of function and loss of function (morpholino oligonucleotides) in chick are outlined, and methods for introducing these reagents by electroporation are detailed.

KW - Chick

KW - Electroporation

KW - Embryo culture

KW - Morpholino oligonucleotide

KW - Gene Transfer Techniques

KW - Gene Expression

KW - Signal Transduction

KW - Embryo Culture Techniques

KW - Chick Embryo

KW - Gene Knockdown Techniques

KW - Animals

KW - Genetic Engineering

KW - Gene Expression Regulation, Developmental

KW - Embryonic Development/genetics

KW - Transgenes

KW - Genes, Reporter

UR - https://www.scopus.com/pages/publications/85061288783

UR - https://www.scopus.com/inward/citedby.url?scp=85061288783&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-9009-2_6

DO - 10.1007/978-1-4939-9009-2_6

M3 - Chapter

C2 - 30737687

AN - SCOPUS:85061288783

VL - 1920

T3 - Methods in Molecular Biology

SP - 75

EP - 97

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Embryological and genetic manipulation of chick development

Abstract

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