Embryological and genetic manipulation of chick development

Laura S Gammill, Bridget Jacques-Fricke, Julaine Roffers-Agarwal

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

The ability to combine embryological manipulations with gene function analysis in an amniote embryo makes the chick a valuable system for the vertebrate developmental biologist. This chapter describes methods for those unfamiliar with the chick system wishing to initiate experiments in their lab. After outlining methods to prepare chick embryos, protocols are provided for introducing beads or cells expressing secreted factors, and for culturing tissue explants as a means of assessing development in vitro. Approaches to achieve gain of function and loss of function (morpholino oligonucleotides) in chick are outlined, and methods for introducing these reagents by electroporation are detailed.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages75-97
Number of pages23
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1920
ISSN (Print)1064-3745

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Keywords

  • Chick
  • Electroporation
  • Embryo culture
  • Morpholino oligonucleotide

PubMed: MeSH publication types

  • Journal Article

Cite this

Gammill, L. S., Jacques-Fricke, B., & Roffers-Agarwal, J. (2019). Embryological and genetic manipulation of chick development. In Methods in Molecular Biology (pp. 75-97). (Methods in Molecular Biology; Vol. 1920). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9009-2_6