Embryological and genetic manipulation of chick development

Laura S. Gammill, Catherine E. Krull

Research output: Chapter in Book/Report/Conference proceedingChapter

11 Scopus citations

Abstract

The ability to combine embryological manipulations with gene function analysis makes the chick a valuable system for the vertebrate developmental biologist. We describe methods for those unfamiliar with the chick wishing to initiate chick experiments in their lab. After outlining how to prepare chick embryos, we provide protocols for introducing beads or cells expressing secreted factors into the embryo and for culturing tissue explants as a means of assessing development in vitro. Chick gain-of-function and loss-of-function (RNAi and morpholino oligonucleotide) approaches are outlined, and methods for introducing these reagents by electroporation are detailed.

Original languageEnglish (US)
Title of host publicationVertebrate Embryogenesis
Subtitle of host publicationEmbryological, Cellular, and Genetic Methods
EditorsFrancisco Pelegri
Pages119-137
Number of pages19
DOIs
StatePublished - Sep 23 2011

Publication series

NameMethods in Molecular Biology
Volume770
ISSN (Print)1064-3745

Keywords

  • Chick
  • RNAi
  • electroporation
  • embryology
  • morpholino oligonucleotide

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