Elevated levels of recombinational DNA repair in human somatic cells expressing the Saccharomyces cerevisiae RAD52 gene

Brad L. Johnson; Bhaskar Thyagarajan; Lisa Krueger; Betsy A Hirsch; Colin R Campbell

doi:10.1016/0921-8777(96)00007-9

Elevated levels of recombinational DNA repair in human somatic cells expressing the Saccharomyces cerevisiae RAD52 gene

Brad L. Johnson, Bhaskar Thyagarajan, Lisa Krueger, Betsy A Hirsch, Colin R Campbell

Research output: Contribution to journal › Article › peer-review

23 Scopus citations

Abstract

The Saccharomyces cerevisiae RAD52 gene was introduced into the human fibrosarcoma-derived cell line HT1080. Transfected cell lines that expressed the yeast transgene catalyzed inter-plasmid homologous DNA recombination at frequencies approx. 12-fold higher than did control cells. Additional experiments revealed that yeast RAD52 gene expression increased the level of resistance to the DNA damaging agents diepoxybutane, and methyl methanesulfonate, but did not alter sensitivity to ultraviolet radiation. These results indicate that the S. cerevisiae Rad52 protein can function in a human somatic cell background and provide support for the idea that a homologous recombination-based DNA repair process functions in mammalian somatic cells.

Original language	English (US)
Pages (from-to)	179-189
Number of pages	11
Journal	Mutation Research - DNA Repair
Volume	363
Issue number	3
DOIs	https://doi.org/10.1016/0921-8777(96)00007-9
State	Published - Aug 8 1996

Bibliographical note

Funding Information:
This work was supported in part by funds provided by funds provided by the Minnesota Medical Foundation, the H. Louise Ruddell Charitable Trust, the American Heart Association, MN Affiliate (to C.C.) and by NIH grant number AGO6886 (B.H.). B.L.J. was supportedt hrough funds provided by the University of Minnesota Graduate School. We thank Dr. Cecilia Warner for helpful editorial comments.

Keywords

Homologous recombination
Human somatic cell
Yeast RAD52 gene

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title = "Elevated levels of recombinational DNA repair in human somatic cells expressing the Saccharomyces cerevisiae RAD52 gene",

abstract = "The Saccharomyces cerevisiae RAD52 gene was introduced into the human fibrosarcoma-derived cell line HT1080. Transfected cell lines that expressed the yeast transgene catalyzed inter-plasmid homologous DNA recombination at frequencies approx. 12-fold higher than did control cells. Additional experiments revealed that yeast RAD52 gene expression increased the level of resistance to the DNA damaging agents diepoxybutane, and methyl methanesulfonate, but did not alter sensitivity to ultraviolet radiation. These results indicate that the S. cerevisiae Rad52 protein can function in a human somatic cell background and provide support for the idea that a homologous recombination-based DNA repair process functions in mammalian somatic cells.",

keywords = "Homologous recombination, Human somatic cell, Yeast RAD52 gene",

author = "Johnson, {Brad L.} and Bhaskar Thyagarajan and Lisa Krueger and Hirsch, {Betsy A} and Campbell, {Colin R}",

note = "Funding Information: This work was supported in part by funds provided by funds provided by the Minnesota Medical Foundation, the H. Louise Ruddell Charitable Trust, the American Heart Association, MN Affiliate (to C.C.) and by NIH grant number AGO6886 (B.H.). B.L.J. was supportedt hrough funds provided by the University of Minnesota Graduate School. We thank Dr. Cecilia Warner for helpful editorial comments.",

year = "1996",

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doi = "10.1016/0921-8777(96)00007-9",

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T1 - Elevated levels of recombinational DNA repair in human somatic cells expressing the Saccharomyces cerevisiae RAD52 gene

AU - Johnson, Brad L.

AU - Thyagarajan, Bhaskar

AU - Krueger, Lisa

AU - Hirsch, Betsy A

AU - Campbell, Colin R

N1 - Funding Information: This work was supported in part by funds provided by funds provided by the Minnesota Medical Foundation, the H. Louise Ruddell Charitable Trust, the American Heart Association, MN Affiliate (to C.C.) and by NIH grant number AGO6886 (B.H.). B.L.J. was supportedt hrough funds provided by the University of Minnesota Graduate School. We thank Dr. Cecilia Warner for helpful editorial comments.

PY - 1996/8/8

Y1 - 1996/8/8

N2 - The Saccharomyces cerevisiae RAD52 gene was introduced into the human fibrosarcoma-derived cell line HT1080. Transfected cell lines that expressed the yeast transgene catalyzed inter-plasmid homologous DNA recombination at frequencies approx. 12-fold higher than did control cells. Additional experiments revealed that yeast RAD52 gene expression increased the level of resistance to the DNA damaging agents diepoxybutane, and methyl methanesulfonate, but did not alter sensitivity to ultraviolet radiation. These results indicate that the S. cerevisiae Rad52 protein can function in a human somatic cell background and provide support for the idea that a homologous recombination-based DNA repair process functions in mammalian somatic cells.

AB - The Saccharomyces cerevisiae RAD52 gene was introduced into the human fibrosarcoma-derived cell line HT1080. Transfected cell lines that expressed the yeast transgene catalyzed inter-plasmid homologous DNA recombination at frequencies approx. 12-fold higher than did control cells. Additional experiments revealed that yeast RAD52 gene expression increased the level of resistance to the DNA damaging agents diepoxybutane, and methyl methanesulfonate, but did not alter sensitivity to ultraviolet radiation. These results indicate that the S. cerevisiae Rad52 protein can function in a human somatic cell background and provide support for the idea that a homologous recombination-based DNA repair process functions in mammalian somatic cells.

KW - Homologous recombination

KW - Human somatic cell

KW - Yeast RAD52 gene

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AN - SCOPUS:0030575549

SN - 0921-8777

VL - 363

SP - 179

EP - 189

JO - Mutation Research - DNA Repair

JF - Mutation Research - DNA Repair

IS - 3

ER -

Elevated levels of recombinational DNA repair in human somatic cells expressing the Saccharomyces cerevisiae RAD52 gene

Abstract

Bibliographical note

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