The Saccharomyces cerevisiae RAD52 gene was introduced into the human fibrosarcoma-derived cell line HT1080. Transfected cell lines that expressed the yeast transgene catalyzed inter-plasmid homologous DNA recombination at frequencies approx. 12-fold higher than did control cells. Additional experiments revealed that yeast RAD52 gene expression increased the level of resistance to the DNA damaging agents diepoxybutane, and methyl methanesulfonate, but did not alter sensitivity to ultraviolet radiation. These results indicate that the S. cerevisiae Rad52 protein can function in a human somatic cell background and provide support for the idea that a homologous recombination-based DNA repair process functions in mammalian somatic cells.
Bibliographical noteFunding Information:
This work was supported in part by funds provided by funds provided by the Minnesota Medical Foundation, the H. Louise Ruddell Charitable Trust, the American Heart Association, MN Affiliate (to C.C.) and by NIH grant number AGO6886 (B.H.). B.L.J. was supportedt hrough funds provided by the University of Minnesota Graduate School. We thank Dr. Cecilia Warner for helpful editorial comments.
- Homologous recombination
- Human somatic cell
- Yeast RAD52 gene