Elevated antioxidant potential of chlorocholine chloride-treated in vitro grown Stevia rebaudiana Bertoni

Avishek Dey, Souren Paul, Sayanti Kundu, Abhijit Bandyopadhyay, Aloke Bhattacharjee

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


Medicinal plants contain a plethora of secondary metabolites, most of which are bioactive in nature. The role of a popular plant growth retardant CCC has been investigated to explore its impact on secondary metabolite production, particularly phenols and flavonoids from in vitro grown Stevia rebaudiana. CCC stimulated the production of total phenols and flavonoids in calli and leaves. Moreover, this elevated level of phenols and flavonoids was correlated with the antioxidant potential of the tissue extracts. Methanolic extracts from CCC-treated calli and leaves showed significant increment in antioxidant activity as determined by standard DPPH, ABTS, and hydroxyl radical scavenging assays. No significant antiproliferative effect of methanolic extracts from different tissue was noticed against THP-1 monocyte (ATCC-TIB202), Hela cell (ATCC-CCL2) lines endorses the issue of clinical safety of the extracts.

Original languageEnglish (US)
Pages (from-to)1775-1783
Number of pages9
JournalActa Physiologiae Plantarum
Issue number6
StatePublished - Jun 2013
Externally publishedYes

Bibliographical note

Funding Information:
Acknowledgments The authors gratefully acknowledge their respected supervisors for valuable and constructive suggestions. They are also thankful to the Head, Department of Botany, The University of Burdwan, for providing laboratory facilities. Financial assistance from University Grant Commission, New Delhi, is duly acknowledged.


  • Antioxidant activities
  • Chlorocholine chloride (CCC)
  • Cytotoxicity
  • Stevia rebaudiana


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