Elementary mode analysis: A useful metabolic pathway analysis tool for characterizing cellular metabolism

Cong T. Trinh, Aaron Wlaschin, Friedrich Srienc

Research output: Contribution to journalShort surveypeer-review

189 Scopus citations

Abstract

Elementary mode analysis is a useful metabolic pathway analysis tool to identify the structure of a metabolic network that links the cellular phenotype to the corresponding genotype. The analysis can decompose the intricate metabolic network comprised of highly interconnected reactions into uniquely organized pathways. These pathways consisting of a minimal set of enzymes that can support steady state operation of cellular metabolism represent independent cellular physiological states. Such pathway definition provides a rigorous basis to systematically characterize cellular phenotypes, metabolic network regulation, robustness, and fragility that facilitate understanding of cell physiology and implementation of metabolic engineering strategies. This mini-review aims to overview the development and application of elementary mode analysis as a metabolic pathway analysis tool in studying cell physiology and as a basis of metabolic engineering.

Original languageEnglish (US)
Pages (from-to)813-826
Number of pages14
JournalApplied Microbiology and Biotechnology
Volume81
Issue number5
DOIs
StatePublished - Jan 2009

Keywords

  • Control effective flux
  • Elementary mode
  • Extreme pathway
  • Genetic knockout analysis
  • Metabolic engineering
  • Metabolic flux ratio
  • Metabolic pathway analysis
  • Minimal cell
  • Minimal cut set
  • Rational strain design
  • Robustness
  • Weighting factors

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