Elemental Concentrations in the Seed of Mutants and Natural Variants of Arabidopsis thaliana Grown under Varying Soil Conditions

Stephen C. McDowell, Garo Akmakjian, Chris Sladek, David Mendoza-Cozatl, Joe B. Morrissey, Nick Saini, Ron Mittler, Ivan Baxter, David E. Salt, John M Ward, Julian I. Schroeder, Mary Lou Guerinot, Jeffrey F. Harper

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The concentrations of mineral nutrients in seeds are critical to both the life cycle of plants as well as human nutrition. These concentrations are strongly influenced by soil conditions, as shown here by quantifying the concentration of 14 elements in seeds from Arabidopsis thaliana plants grown under four different soil conditions: standard, or modified with NaCl, heavy metals, or alkali. Each of the modified soils resulted in a unique change to the seed ionome (the mineral nutrient content of the seeds). To help identify the genetic networks regulating the seed ionome, changes in elemental concentrations were evaluated using mutants corresponding to 760 genes as well as 10 naturally occurring accessions. The frequency of ionomic phenotypes supports an estimate that as much as 11% of the A. thaliana genome encodes proteins of functional relevance to ion homeostasis in seeds. A subset of mutants were analyzed with two independent alleles, providing five examples of genes important for regulation of the seed ionome: SOS2, ABH1, CCC, At3g14280 and CNGC2. In a comparison of nine different accessions to a Col-0 reference, eight accessions were observed to have reproducible differences in elemental concentrations, seven of which were dependent on specific soil conditions. These results indicate that the A. thaliana seed ionome is distinct from the vegetative ionome, and that elemental analysis is a sensitive approach to identify genes controlling ion homeostasis, including those that regulate gene expression, phospho-regulation, and ion transport.

Original languageEnglish (US)
Article numbere63014
JournalPloS one
Volume8
Issue number5
DOIs
StatePublished - May 6 2013

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Arabidopsis
Seed
soil quality
Seeds
Soil
Arabidopsis thaliana
Soils
mutants
seeds
Genes
Ions
Nutrients
Minerals
homeostasis
Homeostasis
ions
Food
genes
Ion Transport
human nutrition

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McDowell, S. C., Akmakjian, G., Sladek, C., Mendoza-Cozatl, D., Morrissey, J. B., Saini, N., ... Harper, J. F. (2013). Elemental Concentrations in the Seed of Mutants and Natural Variants of Arabidopsis thaliana Grown under Varying Soil Conditions. PloS one, 8(5), [e63014]. https://doi.org/10.1371/journal.pone.0063014

Elemental Concentrations in the Seed of Mutants and Natural Variants of Arabidopsis thaliana Grown under Varying Soil Conditions. / McDowell, Stephen C.; Akmakjian, Garo; Sladek, Chris; Mendoza-Cozatl, David; Morrissey, Joe B.; Saini, Nick; Mittler, Ron; Baxter, Ivan; Salt, David E.; Ward, John M; Schroeder, Julian I.; Guerinot, Mary Lou; Harper, Jeffrey F.

In: PloS one, Vol. 8, No. 5, e63014, 06.05.2013.

Research output: Contribution to journalArticle

McDowell, SC, Akmakjian, G, Sladek, C, Mendoza-Cozatl, D, Morrissey, JB, Saini, N, Mittler, R, Baxter, I, Salt, DE, Ward, JM, Schroeder, JI, Guerinot, ML & Harper, JF 2013, 'Elemental Concentrations in the Seed of Mutants and Natural Variants of Arabidopsis thaliana Grown under Varying Soil Conditions', PloS one, vol. 8, no. 5, e63014. https://doi.org/10.1371/journal.pone.0063014
McDowell, Stephen C. ; Akmakjian, Garo ; Sladek, Chris ; Mendoza-Cozatl, David ; Morrissey, Joe B. ; Saini, Nick ; Mittler, Ron ; Baxter, Ivan ; Salt, David E. ; Ward, John M ; Schroeder, Julian I. ; Guerinot, Mary Lou ; Harper, Jeffrey F. / Elemental Concentrations in the Seed of Mutants and Natural Variants of Arabidopsis thaliana Grown under Varying Soil Conditions. In: PloS one. 2013 ; Vol. 8, No. 5.
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