Electropherotypic analysis of rotaviruses isolated from turkeys.

S. Y. Kang, K. V. Nagaraja, J. A. Newman

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


From 1980 to 1984, several flocks of turkeys in Minnesota exhibiting signs of clinical enteritis were examined for viruses. Electron microscopic (EM) examination of fecal specimens from 35 flocks revealed the presence of rotavirus particles. Rotaviruses were successfully isolated in cell cultures from only 24 of these positive fecal specimens. Double-stranded RNA (dsRNA) preparations made from these 24 cell-culture isolates and from the remaining 11 fecal samples that were rotavirus-positive on EM examination were analyzed by polyacrylamide gel electrophoresis for genetic differences in their genomes. The study revealed eight distinct electropherotypes among the rotavirus dsRNA preparations. Atypical dsRNA migration patterns were recognized only in preparations of dsRNA from fecal materials.

Original languageEnglish (US)
Pages (from-to)794-801
Number of pages8
JournalAvian diseases
Issue number4
StatePublished - 1986


Dive into the research topics of 'Electropherotypic analysis of rotaviruses isolated from turkeys.'. Together they form a unique fingerprint.

Cite this