Abstract
We have used site-directed spin labeling and electron paramagnetic resonance (EPR) to explore the effects of oxidation on muscle function, with particular focus on the actin-myosin interaction. EPR measurements show that aging or oxidative modification causes a decrease in the fraction of myosins in the strong-binding state, which can be traced to the actin-binding cleft of the myosin catalytic domain.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 30-36 |
| Number of pages | 7 |
| Journal | Exercise and sport sciences reviews |
| Volume | 42 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2014 |
Keywords
- ROS
- actin
- aging
- methionine
- muscle
- myosin
- reactive oxygen species