Electron microscopy and image analysis of the GroEL-l protein and its complexes with glutamine synthe from pea leaves

Vladimir L. Tsuprun; Egbert J. Boekema; Alexander V. Pushkin; Irina V. Tagunowa

doi:10.1016/0005-2728(92)90188-8

Electron microscopy and image analysis of the GroEL-l protein and its complexes with glutamine synthe from pea leaves

Vladimir L. Tsuprun, Egbert J. Boekema, Alexander V. Pushkin, Irina V. Tagunowa

Otolaryngology

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Abstract

The molecular structure of GroEL-like protein from pea leaves has been studied by electron microscopy and image analysis of negatively stained particles. Over 1500 molecular projections were selected and classified by multivariate statistical analysis. It was shown that the molecule consists of 14 subunits arranged in two layers with 72 point group symmetry. Side view projections of the molecule show a four-striation appearance, which subdivides both layers of seven subunits into two halves: this may be explained by a two-domain structure of the subunits. The presence in protein preparations of projections corresponding to one layer of subunits of half-molecules is consistent with the molecular structure suggested. Electron microscopic evidence for a specific association of GroEL-like protein and octameric glutamine synthetase, which was co-purified with this protein, was obtained.

Original language	English (US)
Pages (from-to)	67-73
Number of pages	7
Journal	BBA - Bioenergetics
Volume	1099
Issue number	1
DOIs	https://doi.org/10.1016/0005-2728(92)90188-8
State	Published - Jan 30 1992

Bibliographical note

Funding Information:
We wish to thank Prof.N .A. Kisclev and Prof. W.L. Kretovich for helpful discussionsD, r. W+ Keegstraf or help with computerim age analysis anMdr . K. Gilissen for technicala ssistanceT. he research of E.J.B. has been made possible by a fellowship of the Royal NetherlandsA cademyo' f Arts and Sciences.

Keywords

Glutamine synthetase
GroEL-like protein
Image analysis (PEA)
Protein structure, Protein-protein interaction, Electron microscopy

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10.1016/0005-2728(92)90188-8

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title = "Electron microscopy and image analysis of the GroEL-l protein and its complexes with glutamine synthe from pea leaves",

abstract = "The molecular structure of GroEL-like protein from pea leaves has been studied by electron microscopy and image analysis of negatively stained particles. Over 1500 molecular projections were selected and classified by multivariate statistical analysis. It was shown that the molecule consists of 14 subunits arranged in two layers with 72 point group symmetry. Side view projections of the molecule show a four-striation appearance, which subdivides both layers of seven subunits into two halves: this may be explained by a two-domain structure of the subunits. The presence in protein preparations of projections corresponding to one layer of subunits of half-molecules is consistent with the molecular structure suggested. Electron microscopic evidence for a specific association of GroEL-like protein and octameric glutamine synthetase, which was co-purified with this protein, was obtained.",

keywords = "Glutamine synthetase, GroEL-like protein, Image analysis (PEA), Protein structure, Protein-protein interaction, Electron microscopy",

author = "Tsuprun, {Vladimir L.} and Boekema, {Egbert J.} and Pushkin, {Alexander V.} and Tagunowa, {Irina V.}",

note = "Funding Information: We wish to thank Prof.N .A. Kisclev and Prof. W.L. Kretovich for helpful discussionsD, r. W+ Keegstraf or help with computerim age analysis anMdr . K. Gilissen for technicala ssistanceT. he research of E.J.B. has been made possible by a fellowship of the Royal NetherlandsA cademyo' f Arts and Sciences.",

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doi = "10.1016/0005-2728(92)90188-8",

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T1 - Electron microscopy and image analysis of the GroEL-l protein and its complexes with glutamine synthe from pea leaves

AU - Tsuprun, Vladimir L.

AU - Boekema, Egbert J.

AU - Pushkin, Alexander V.

AU - Tagunowa, Irina V.

N1 - Funding Information: We wish to thank Prof.N .A. Kisclev and Prof. W.L. Kretovich for helpful discussionsD, r. W+ Keegstraf or help with computerim age analysis anMdr . K. Gilissen for technicala ssistanceT. he research of E.J.B. has been made possible by a fellowship of the Royal NetherlandsA cademyo' f Arts and Sciences.

PY - 1992/1/30

Y1 - 1992/1/30

N2 - The molecular structure of GroEL-like protein from pea leaves has been studied by electron microscopy and image analysis of negatively stained particles. Over 1500 molecular projections were selected and classified by multivariate statistical analysis. It was shown that the molecule consists of 14 subunits arranged in two layers with 72 point group symmetry. Side view projections of the molecule show a four-striation appearance, which subdivides both layers of seven subunits into two halves: this may be explained by a two-domain structure of the subunits. The presence in protein preparations of projections corresponding to one layer of subunits of half-molecules is consistent with the molecular structure suggested. Electron microscopic evidence for a specific association of GroEL-like protein and octameric glutamine synthetase, which was co-purified with this protein, was obtained.

AB - The molecular structure of GroEL-like protein from pea leaves has been studied by electron microscopy and image analysis of negatively stained particles. Over 1500 molecular projections were selected and classified by multivariate statistical analysis. It was shown that the molecule consists of 14 subunits arranged in two layers with 72 point group symmetry. Side view projections of the molecule show a four-striation appearance, which subdivides both layers of seven subunits into two halves: this may be explained by a two-domain structure of the subunits. The presence in protein preparations of projections corresponding to one layer of subunits of half-molecules is consistent with the molecular structure suggested. Electron microscopic evidence for a specific association of GroEL-like protein and octameric glutamine synthetase, which was co-purified with this protein, was obtained.

KW - Glutamine synthetase

KW - GroEL-like protein

KW - Image analysis (PEA)

KW - Protein structure, Protein-protein interaction, Electron microscopy

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Electron microscopy and image analysis of the GroEL-l protein and its complexes with glutamine synthe from pea leaves

Abstract

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Keywords

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