Electron-microscopic studies on location of SH-groups in mitochondrial F1-ATPase using a ferritin label

Vladimir L. Tsuprun, Irina V. Mesyanzhinova, Yakov M. Milgrom, Tatyana Yu Kalashnikova

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A new approach has been suggested for electron-microscopic study of the structure of mitochondrial F1-ATPase based on ferritin labeling. By means of sequential treatment with 2-iminothiolane and Nbs2 we obtained a modified ferritin (NbsSPrCNH-Ft) able to react with SH-groups of proteins and to form conjugates in which the protein and ferritin are bound by disulfide bonds. An electron-microscopic investigation of the negatively stained preparations of mitochondrial F1-ATPase, preincubated with modified ferritin, revealed such enzyme-ferritin conjugates. In case of modified ferritin, containing 360 mol SH-groups per mol protein, and F1-ATPase, pretreated with N-ethylmaleimide and then with dithiothreitol, conjugates were obtained in which ferritin molecules are bound to several (as many as four) of the six protein masses, comprising a bilayer molecule of the enzyme. Taking into consideration the biochemical data on the location of accessible SH-groups (only in α, γ or ε subunits), it is inferred from the results obtained that one of the protein masses is a complex between β subunit and at least one of the minor subunits located partially on the molecule's external side. This indicates the nonequivalence of different copies of the major subunits. Averaged images of the particles of the F1-F0 complex from bovine heart mitochondria and bacteria Micrococcus lysodeicticus were obtained. It was found that F0 component is bound to two adjacent protein masses of the F1-ATPase molecule. It is suggested that this binding may be due the nonequivalency of single-type major subunits.

Original languageEnglish (US)
Pages (from-to)130-137
Number of pages8
JournalBBA - Bioenergetics
Volume892
Issue number1
DOIs
StatePublished - Jun 9 1987

Keywords

  • ATPase, F-
  • Electron microscopy
  • Enzyme structure
  • Ferritin labelling
  • Sulfhydryl group

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