Electron immunoperoxidase staining in sections of unfixed pure cultures of streptococcus mutans

Peter Berthold; C. H. Berthold; C. E. Nord

doi:10.3109/00016358209024071

Electron immunoperoxidase staining in sections of unfixed pure cultures of streptococcus mutans

Peter Berthold, C. H. Berthold, C. E. Nord

Dental Public Health

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Unfixed as well as glutaraldehyde-fixed pelleted cells from pure cultures of Streptococcus mutans subspecies sobrinus (B13) were embedded in glycolme-thacrylate. Uitrathin were sections immunoperoxidase labelled with rabbit anti-S. mutans sobrinus γglobulin. It was found that unfixed specimens became labelled as precisely and as distinctly as did the fixed specimens. All controls were negative.

Original language	English (US)
Pages (from-to)	283-287
Number of pages	5
Journal	Acta Odontologica Scandinavica
Volume	40
Issue number	5
DOIs	https://doi.org/10.3109/00016358209024071
State	Published - 1982

Bibliographical note

Funding Information:
This work was carried out with financial support from Odontological Faculty at Karolinska Institutet, Stockholm, Sweden and from the Swedish Medical Research Council (project No. 03157). Dr. P. Berthold is currently a post-doctoral fellow in Periodontics supported by NIDR Grant DE 07085.

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

Electron microscopy
Fixation
Immunohistochemistry

Publisher link

10.3109/00016358209024071

Find It

Find It at U of M Libraries

Cite this

@article{bbae1fc8ce62446fb99c97e8d37b4818,

title = "Electron immunoperoxidase staining in sections of unfixed pure cultures of streptococcus mutans",

abstract = "Unfixed as well as glutaraldehyde-fixed pelleted cells from pure cultures of Streptococcus mutans subspecies sobrinus (B13) were embedded in glycolme-thacrylate. Uitrathin were sections immunoperoxidase labelled with rabbit anti-S. mutans sobrinus γglobulin. It was found that unfixed specimens became labelled as precisely and as distinctly as did the fixed specimens. All controls were negative.",

keywords = "Electron microscopy, Fixation, Immunohistochemistry",

author = "Peter Berthold and Berthold, {C. H.} and Nord, {C. E.}",

note = "Funding Information: This work was carried out with financial support from Odontological Faculty at Karolinska Institutet, Stockholm, Sweden and from the Swedish Medical Research Council (project No. 03157). Dr. P. Berthold is currently a post-doctoral fellow in Periodontics supported by NIDR Grant DE 07085. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1982",

doi = "10.3109/00016358209024071",

language = "English (US)",

volume = "40",

pages = "283--287",

journal = "Acta Odontologica Scandinavica",

issn = "0001-6357",

publisher = "Informa Healthcare",

number = "5",

}

TY - JOUR

T1 - Electron immunoperoxidase staining in sections of unfixed pure cultures of streptococcus mutans

AU - Berthold, Peter

AU - Berthold, C. H.

AU - Nord, C. E.

N1 - Funding Information: This work was carried out with financial support from Odontological Faculty at Karolinska Institutet, Stockholm, Sweden and from the Swedish Medical Research Council (project No. 03157). Dr. P. Berthold is currently a post-doctoral fellow in Periodontics supported by NIDR Grant DE 07085. Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1982

Y1 - 1982

N2 - Unfixed as well as glutaraldehyde-fixed pelleted cells from pure cultures of Streptococcus mutans subspecies sobrinus (B13) were embedded in glycolme-thacrylate. Uitrathin were sections immunoperoxidase labelled with rabbit anti-S. mutans sobrinus γglobulin. It was found that unfixed specimens became labelled as precisely and as distinctly as did the fixed specimens. All controls were negative.

AB - Unfixed as well as glutaraldehyde-fixed pelleted cells from pure cultures of Streptococcus mutans subspecies sobrinus (B13) were embedded in glycolme-thacrylate. Uitrathin were sections immunoperoxidase labelled with rabbit anti-S. mutans sobrinus γglobulin. It was found that unfixed specimens became labelled as precisely and as distinctly as did the fixed specimens. All controls were negative.

KW - Electron microscopy

KW - Fixation

KW - Immunohistochemistry

UR - http://www.scopus.com/inward/record.url?scp=0020334101&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020334101&partnerID=8YFLogxK

U2 - 10.3109/00016358209024071

DO - 10.3109/00016358209024071

M3 - Article

C2 - 6758470

AN - SCOPUS:0020334101

SN - 0001-6357

VL - 40

SP - 283

EP - 287

JO - Acta Odontologica Scandinavica

JF - Acta Odontologica Scandinavica

IS - 5

ER -

Electron immunoperoxidase staining in sections of unfixed pure cultures of streptococcus mutans

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this