Unfixed as well as glutaraldehyde-fixed pelleted cells from pure cultures of Streptococcus mutans subspecies sobrinus (B13) were embedded in glycolme-thacrylate. Uitrathin were sections immunoperoxidase labelled with rabbit anti-S. mutans sobrinus γglobulin. It was found that unfixed specimens became labelled as precisely and as distinctly as did the fixed specimens. All controls were negative.
Bibliographical noteFunding Information:
This work was carried out with financial support from Odontological Faculty at Karolinska Institutet, Stockholm, Sweden and from the Swedish Medical Research Council (project No. 03157). Dr. P. Berthold is currently a post-doctoral fellow in Periodontics supported by NIDR Grant DE 07085.
Copyright 2014 Elsevier B.V., All rights reserved.
- Electron microscopy