Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G

Junya Chiba; Takahide Kouno; Shun Aoki; Hitoshi Sato; Jingying Zhang; Hiroshi Matsuo; Masahiko Inouye

doi:10.1039/c2cc36779c

Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G

Junya Chiba, Takahide Kouno, Shun Aoki, Hitoshi Sato, Jingying Zhang, Hiroshi Matsuo, Masahiko Inouye

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Abstract

APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.

Original language	English (US)
Pages (from-to)	12115-12117
Number of pages	3
Journal	Chemical Communications
Volume	48
Issue number	99
DOIs	https://doi.org/10.1039/c2cc36779c
State	Published - Nov 19 2012

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title = "Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G",

abstract = "APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.",

author = "Junya Chiba and Takahide Kouno and Shun Aoki and Hitoshi Sato and Jingying Zhang and Hiroshi Matsuo and Masahiko Inouye",

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T1 - Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G

AU - Chiba, Junya

AU - Kouno, Takahide

AU - Aoki, Shun

AU - Sato, Hitoshi

AU - Zhang, Jingying

AU - Matsuo, Hiroshi

AU - Inouye, Masahiko

PY - 2012/11/19

Y1 - 2012/11/19

N2 - APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.

AB - APOBEC3G catalyzes deamination of cytosines in HIV-1 genome, and restricts the HIV-1 infection. Here, we propose a picomole-scale assay for the detection of DNA deamination catalyzed by APOBEC3G. Our results show the suitability of the developed method for a time course analysis of enzyme-catalyzed DNA modifications.

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JO - Chemical Communications

JF - Chemical Communications

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ER -

Electrochemical direct detection of DNA deamination catalyzed by APOBEC3G

Abstract

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