Electrochemical approach to detect apoptosis

Han Xiao, Lei Liu, Fanben Meng, Junyi Huang, Genxi Li

Research output: Contribution to journalArticlepeer-review

75 Scopus citations

Abstract

This paper reports an electrochemical approach for detection of apoptosis. Here we prepare a gold electrode modified with a helix peptide ferrocene (Fc)-GDGDEVDGC. Fc is used as an electroactive reporter and the peptide as a recognition and cleavage site of caspase-3, which is a special proteinase to apoptosis. Results show that this method may sensitively and specifically detect apoptotic cells with signal decline of 85%. This approach is different from the previous methods for apoptosis detection, because it does not need any fluorescent materials, expensive biological instruments, or complicated procedures.

Original languageEnglish (US)
Pages (from-to)5272-5275
Number of pages4
JournalAnalytical chemistry
Volume80
Issue number13
DOIs
StatePublished - Jul 1 2008

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