Elastomeric polypentapeptides cross-linked into matrixes and fibers

J. Lee, Chris Macosko, D. W. Urry

Research output: Contribution to journalArticle

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Abstract

Microbially prepared polypentapeptides were cross-linked by two chemical methods. In one chemical approach, (GVGIP)260 where G = glycine, V = valine, I = isoleucine, and P = proline with no functional groups in its side chains, was cross-linked using dicumyl peroxide, and reaction conditions were systematically examined. Successful cross-linking was obtained even under severe conditions for proteins, i.e., 3 h at 120 °C, without having significant side reactions. In the second chemical approach, two separate polymers, (GVGVP GVGVP GXGVP GVGVP GVGVP GVGVP)n where X is either E (the carboxylic acid containing glutamic acid residue) or K (the lysine residue with an ε-amino function), were mixed and cross-linked using a carbodiimde reagent. The reaction temperature was found to affect the equilibrium swelling behavior of the resulting cross-linked hydrogels. In hydrogels cross-linked at a temperature above their hydrophobic folding and assembling transition temperature, 3D continuous filamentous microstructures were observed. Chemically cross-linked hydrogel fibers were also prepared and their anisotropy in swelling was confirmed. Uniaxial tensile moduli and equilibrium weight swelling ratios of the chemically cross-linked samples were compared to those of (GVGVP)251 and (GVGIP)260, γ-irradiation cross-linked at different Mrad doses.

Original languageEnglish (US)
Pages (from-to)170-179
Number of pages10
JournalBiomacromolecules
Volume2
Issue number1
DOIs
StatePublished - Aug 15 2001

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Hydrogels
Swelling
Fibers
Isoleucine
Hydrogel
Valine
Peroxides
Carboxylic Acids
Carboxylic acids
Proline
Glycine
Functional groups
Superconducting transition temperature
Lysine
Dosimetry
Amino acids
Glutamic Acid
Polymers
Anisotropy
Elastic moduli

Cite this

Elastomeric polypentapeptides cross-linked into matrixes and fibers. / Lee, J.; Macosko, Chris; Urry, D. W.

In: Biomacromolecules, Vol. 2, No. 1, 15.08.2001, p. 170-179.

Research output: Contribution to journalArticle

Lee, J. ; Macosko, Chris ; Urry, D. W. / Elastomeric polypentapeptides cross-linked into matrixes and fibers. In: Biomacromolecules. 2001 ; Vol. 2, No. 1. pp. 170-179.
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