Efficient transposition of Tol2 in the mouse germline

Vincent W. Keng, Barbara J. Ryan, Kirk J. Wangensteen, Darius Balciunas, Christian Schmedt, Stephen C. Ekker, David A. Largaespada

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Insertional mutagenesis screens play an integral part in the annotating of functional data for all sequenced genes in the postgenomic era. Chemical mutagenesis screens are highly efficient but identifying the causative gene can be a laborious task. Other mutagenesis platforms, such as transposable elements, have been successfully applied for insertional mutagenesis screens in both the mouse and rat. However, relatively low transposition efficiency has hampered their use as a high-throughput forward genetic mutagenesis screen. Here we report the first evidence of germline activity in the mouse using a naturally active DNA transposon derived from the medaka fish called Tol2, as an alternative system for high-throughput forward genetic mutagenesis screening tool.

Original languageEnglish (US)
Pages (from-to)1565-1573
Number of pages9
JournalGenetics
Volume183
Issue number4
DOIs
StatePublished - Dec 2009

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    Keng, V. W., Ryan, B. J., Wangensteen, K. J., Balciunas, D., Schmedt, C., Ekker, S. C., & Largaespada, D. A. (2009). Efficient transposition of Tol2 in the mouse germline. Genetics, 183(4), 1565-1573. https://doi.org/10.1534/genetics.109.100768