Efficient transformation of Agrobacterium tumefaciens by electroporation

Mark Mersereau, Gregory J. Pazour, Anath Das

Research output: Contribution to journalArticlepeer-review

126 Scopus citations

Abstract

High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 × 108 transformants/μg DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens.

Original languageEnglish (US)
Pages (from-to)149-151
Number of pages3
JournalGene
Volume90
Issue number1
DOIs
StatePublished - May 31 1990

Bibliographical note

Funding Information:
We thankN eilO lszewskfio rt heu seo fthee lectroporator in thiss tudya ndB onnieA llenf ort ypingo ft hem anuscript. This workw ass upportebdy a grant( GM 37555f)r omt he NationaIln stituteosf H ealthA. .D. is a recipienotf a Junior FacultyR esearchA ward (JFRA-170) of the American CanceSr ocietya nda McKnightL and-GranPtr ofessorship of the Universityo f Minnesota.

Keywords

  • Recombinant DNA
  • Ti plasmid
  • methylation
  • phytopathogen
  • restriction system
  • soil bacteria

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