Efficient transformation of Agrobacterium tumefaciens by electroporation

Mark Mersereau; Gregory J. Pazour; Anath Das

doi:10.1016/0378-1119(90)90452-W

Efficient transformation of Agrobacterium tumefaciens by electroporation

Mark Mersereau, Gregory J. Pazour, Anath Das

Molecular Biology (BMBB)

Research output: Contribution to journal › Article › peer-review

126 Scopus citations

Abstract

High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 × 10⁸ transformants/μg DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens.

Original language	English (US)
Pages (from-to)	149-151
Number of pages	3
Journal	Gene
Volume	90
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(90)90452-W
State	Published - May 31 1990

Bibliographical note

Funding Information:
We thankN eilO lszewskfio rt heu seo fthee lectroporator in thiss tudya ndB onnieA llenf ort ypingo ft hem anuscript. This workw ass upportebdy a grant( GM 37555f)r omt he NationaIln stituteosf H ealthA. .D. is a recipienotf a Junior FacultyR esearchA ward (JFRA-170) of the American CanceSr ocietya nda McKnightL and-GranPtr ofessorship of the Universityo f Minnesota.

Keywords

Recombinant DNA
Ti plasmid
methylation
phytopathogen
restriction system
soil bacteria

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10.1016/0378-1119(90)90452-W

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title = "Efficient transformation of Agrobacterium tumefaciens by electroporation",

abstract = "High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 × 108 transformants/μg DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens.",

keywords = "Recombinant DNA, Ti plasmid, methylation, phytopathogen, restriction system, soil bacteria",

author = "Mark Mersereau and Pazour, {Gregory J.} and Anath Das",

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doi = "10.1016/0378-1119(90)90452-W",

language = "English (US)",

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pages = "149--151",

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AU - Mersereau, Mark

AU - Pazour, Gregory J.

AU - Das, Anath

N1 - Funding Information: We thankN eilO lszewskfio rt heu seo fthee lectroporator in thiss tudya ndB onnieA llenf ort ypingo ft hem anuscript. This workw ass upportebdy a grant( GM 37555f)r omt he NationaIln stituteosf H ealthA. .D. is a recipienotf a Junior FacultyR esearchA ward (JFRA-170) of the American CanceSr ocietya nda McKnightL and-GranPtr ofessorship of the Universityo f Minnesota.

PY - 1990/5/31

Y1 - 1990/5/31

N2 - High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 × 108 transformants/μg DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens.

AB - High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 × 108 transformants/μg DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens.

KW - Recombinant DNA

KW - Ti plasmid

KW - methylation

KW - phytopathogen

KW - restriction system

KW - soil bacteria

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JO - Gene

JF - Gene

SN - 0378-1119

IS - 1

ER -

Efficient transformation of Agrobacterium tumefaciens by electroporation

Abstract

Bibliographical note

Keywords

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