High-voltage electroporation was used to transform Agrobacterium tumefaciens strains A136 and A348, reaching the efficiency of 1-3 × 108 transformants/μg DNA. Transformation frequency was dependent on the electrical field strength and the pulse length. No significant reduction in transformation efficiency was observed when the transforming DNA contained sites sensitive to endonuclease AtuCI of A. tumefaciens.
Bibliographical noteFunding Information:
We thankN eilO lszewskfio rt heu seo fthee lectroporator in thiss tudya ndB onnieA llenf ort ypingo ft hem anuscript. This workw ass upportebdy a grant( GM 37555f)r omt he NationaIln stituteosf H ealthA. .D. is a recipienotf a Junior FacultyR esearchA ward (JFRA-170) of the American CanceSr ocietya nda McKnightL and-GranPtr ofessorship of the Universityo f Minnesota.
- Recombinant DNA
- Ti plasmid
- restriction system
- soil bacteria