Efficient organogenesis and plantlet regeneration in the timber species Cunninghamia lanceolata (Lamb.) Hook

Mu Lan Zhu, Ji Wang, Yang Yu, Sheng Jun Liu, Zhi Ming Wei

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Abstract

Two efficient regeneration systems were developed in Cunninghamia lanceolata, the most important conifer for industrial wood production in China. Cotyledons and hypocotyls derived from greenhouse-grown seedlings were used as initial explants in our research. A high frequency (95.1 ± 1.84%) of adventitious buds were initiated directly from cotyledons cultured on Douglas-fir cotyledon revised (DCR) medium supplemented with 1 mg l-1 benzyladenine (BA), 0.1 mg l-1 α-naphthaleneacetic acid (NAA), and 0.004 mg l-1 thidiazuron (TDZ) with a maximum mean number of adventitious buds per cotyledon explant of 3.76 ± 0.08. In contrast, a high percentage (93.73 ± 0.55%) of adventitious buds regenerated via callus produced from hypocotyls cultured on DCR medium supplemented with plant growth regulators with a maximum number of adventitious buds per explant (16.71 ± 0.34). Adventitious buds elongated on DCR medium supplemented with 0.2 mg l-1 BA and 0.02 mg l-1 NAA. After liquid pretreatment with 50 mg l-1 indole-3-butyric acid (IBA), over 95% of the shoots successfully rooted on 1/2 DCR medium supplemented with 0.3 mg l-1 IBA. The innovated systems reported in this study will be useful tools for future genetic manipulation of C. lanceolata and may be adapted for large-scale propagation in other conifers.

Original languageEnglish (US)
Pages (from-to)449-455
Number of pages7
JournalIn Vitro Cellular and Developmental Biology - Plant
Volume43
Issue number5
DOIs
StatePublished - Oct 1 2007

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Keywords

  • Cunninghamia lanceolata
  • In vitro propagation
  • Organogenesis
  • Regeneration
  • Timber biotechnology

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