Efficient Muscle Regeneration by Human PSC-Derived CD82+ ERBB3+ NGFR+ Skeletal Myogenic Progenitors

Ning Xie, Sabrina N. Chu, Cassandra Schultz, Sunny S.K. Chan

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


Differentiation of pluripotent stem cells (PSCs) is a promising approach to obtaining large quantities of skeletal myogenic progenitors for disease modeling and cell-based therapy. However, generating skeletal myogenic cells with high regenerative potential is still challenging. We recently reported that skeletal myogenic progenitors generated from mouse PSC-derived teratomas possess robust regenerative potency. We have also found that teratomas derived from human PSCs contain a skeletal myogenic population. Here, we showed that these human PSC-derived skeletal myogenic progenitors had exceptional engraftability. A combination of cell surface markers, CD82, ERBB3, and NGFR enabled efficient purification of skeletal myogenic progenitors. These cells expressed PAX7 and were able to differentiate into MHC+ multinucleated myotubes. We further discovered that these cells are expandable in vitro. Upon transplantation, the expanded cells formed new dystrophin+ fibers that reconstituted almost ¾ of the total muscle volume, and repopulated the muscle stem cell pool. Our study, therefore, demonstrates the possibility of producing large quantities of engraftable skeletal myogenic cells from human PSCs.

Original languageEnglish (US)
Article number362
Issue number3
StatePublished - Feb 2023

Bibliographical note

Funding Information:
The authors would like to thank Christine Rohlf and Timothy Sundquist for their help with animal husbandry. The study was supported by Regenerative Medicine Minnesota Discovery Science Grant (RMM 102516 001) and University of Minnesota startup and Children’s Discovery—Winefest funds. The monoclonal antibody to PAX7, embryonic MHC, neonatal MHC, MHC, MHC-I, and MHC-IIa were obtained from the Developmental Studies Hybridoma Bank, developed under the auspices of the NICHD and maintained by the University of Iowa.

Publisher Copyright:
© 2023 by the authors.


  • cell therapy
  • muscle stem cells
  • muscular dystrophy
  • myogenic differentiation
  • pluripotent stem cells
  • transplantation

PubMed: MeSH publication types

  • Journal Article
  • Research Support, Non-U.S. Gov't


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