Efficient introduction of phosphorothioates into RNA oligonucleotides by 3-ethoxy-1,2,4-dithiazoline-5-one (EDITH)

Qinghong Xu, George Barany, Robert P. Hammer, Karin Musier-Forsyth

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21 Scopus citations

Abstract

We recently reported on the use of 1,2,4-dithiazolidine-3,5-dione (DtsNH) and 3-ethoxy-1,2,4-dithiazoline-5-one (EDITH) as effective sulfurizing reagents for the preparation of phosphorothioate-containing oligodeoxyribonucleotides. One challenge in automated solid-phase synthesis of phosphorothioate-containing RNA is to develop sulfurization reagents that are effective in the presence of bulky 2'-OH protecting groups. The present study demonstrates that EDITH is exceedingly effective at low concentrations (0.05 M) and short reaction times (2 min) for the automated synthesis of oligoribonucleotides.

Original languageEnglish (US)
Pages (from-to)3643-3644
Number of pages2
JournalNucleic acids research
Volume24
Issue number18
DOIs
StatePublished - 1996

Bibliographical note

Funding Information:
We thank Mr Lin Chen for preparing some of the DtsNH and EDITH used in this work. Financial support from NIH grants GM 28934, 42722 and 43552 to GB; GM 49928 to KMF; and grants

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