Efficient in vivo 31P magnetization transfer approach for noninvasively determining multiple kinetic parameters and metabolic fluxes of ATP metabolism in the human brain

Fei Du, Xiao Hong Zhu, Hongyan Qiao, Xiaoliang Zhang, Wei Chen

Research output: Contribution to journalArticlepeer-review

100 Scopus citations

Abstract

ATP metabolism is controlled mainly by ATP synthase (ATPase) and creatine kinase (CK) reactions that regulate cerebral ATP production, transportation, and utilization. These coupled reactions constitute a chemical exchange metabolic network of PCr↔ATP↔Pi characterized by two forward and two reverse reaction fluxes, which can be studied noninvasively by in vivo 31P MRS combined with magnetization transfer (MT). However, it is still debated whether current MT approaches can precisely determine all of these fluxes. We developed and tested a modified in vivo 31P MT approach based on a multiple single-site saturation (MSS) technique to study the entire PCr↔ATP↔Pi network in human occipital lobe at 7T. Our results reveal that 1) the MSS MT approach can explicitly determine all four reaction fluxes with a minimal number of 31P spectra; 2) the three-spin exchange model accurately determines reverse reaction fluxes, resulting in equal forward and reverse fluxes for both CK and ATPase reactions; and 3) the ATP synthesis rate (8.8 ± 1.9 μmol/g/min, N = 11) measured in the human brain reflects cerebral oxidative phosphorylation. The MSS MT approach should provide an important modality for noninvasively studying the essential roles of ATP metabolism in brain bioenergetics, function, and diseases.

Original languageEnglish (US)
Pages (from-to)103-114
Number of pages12
JournalMagnetic resonance in medicine
Volume57
Issue number1
DOIs
StatePublished - Jan 2007

Keywords

  • Adenosine triphosphate (ATP) metabolism
  • Bioenergetics
  • Brain
  • Chemical exchange
  • In vivo P magnetic resonance spectroscopy (MRS)
  • MT
  • Metabolic flux

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