Efficient Estimation of Protein Binding Constants

John E Connett, A. Gharrett, R. McHugh

Research output: Contribution to journalArticle

Abstract

Assays of cyclic AMP typically employ a Scatchard‐like data analysis to estimate the total binding protein RT and the dissociation constant Kd. The linear regression procedure employed is statistically ad hoc because the response variable (bound counts) occurs on both sides of the Scatchard equation. The alternative analysis presented here is derived from the cyclic AMP binding reaction. The resulting data analysis, employing non‐linear least squares, results in estimators that on the basis of Monte Carlo studies have less bias and greater precision than the Scatchard method.

Original languageEnglish (US)
Pages (from-to)941-946
Number of pages6
JournalBiometrical Journal
Volume27
Issue number8
DOIs
StatePublished - 1985

Keywords

  • Cyclic AMP Assay
  • Monte Carlo Study
  • Non‐linear Least Squares
  • Scatchard Plot

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