Efficient Editing of the CXCR4 Locus Using Cas9 Ribonucleoprotein Complexes Stabilized with Polyglutamic Acid

D. S. Golubev, D. S. Komkov, M. V. Shepelev, D. V. Mazurov, N. A. Kruglova

Research output: Contribution to journalArticlepeer-review

Abstract

Abstract: Gene editing using the CRISPR/Cas9 system provides new opportunities to treat human diseases. Approaches aimed at increasing the efficiency of genome editing are therefore important to develop. To increase the level of editing of the CXCR4 locus, which is a target for gene therapy of HIV infection, the Cas9 protein was modified by introducing additional NLS signals and ribonucleoprotein complexes of Cas9 and guide RNA were stabilized with poly-L-glutamic acid. The approach allowed a 1.8-fold increase in the level of CXCR4 knockout in the CEM/R5 T cell line and a 2-fold increase in the level of knock-in of the HIV-1 fusion peptide inhibitor MT-C34 in primary CD4+ T lymphocytes.

Original languageEnglish (US)
Pages (from-to)S28-S32
JournalDoklady Biological Sciences
Volume513
Issue numberSuppl 1
DOIs
StatePublished - Dec 2023

Bibliographical note

Publisher Copyright:
© Pleiades Publishing, Ltd. 2023. ISSN 0012-4966, Doklady Biological Sciences, 2023, Vol. 513, Suppl. 1, pp. S28–S32. Pleiades Publishing, Ltd., 2023. ISSN 0012-4966, Doklady Biological Sciences, 2024. Pleiades Publishing, Ltd., 2024.

Keywords

  • CRISPR/Cas9
  • CXCR4
  • HIV
  • NLS
  • PGA
  • RNP
  • genome editing

PubMed: MeSH publication types

  • Journal Article

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