Efficacy of the dietary histone deacetylase inhibitor butyrate alone or in combination with vitamin A against proliferation of MCF-7 human breast cancer cells

F. O. Andrade, M. K. Nagamine, A. De Conti, L. M. Chaible, C. C. Fontelles, A. A. Jordão, H. Vannucchi, M. L.Z. Dagli, B. K. Bassoli, F. S. Moreno, T. P. Ong

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12 Scopus citations

Abstract

The combined treatment with histone deacetylase inhibitors (HDACi) and retinoids has been suggested as a potential epigenetic strategy for the control of cancer. In the present study, we investigated the effects of treatment with butyrate, a dietary HDACi, combined with vitamin A on MCF-7 human breast cancer cells. Cell proliferation was evaluated by the crystal violet staining method. MCF-7 cells were plated at 5 x 104 cells/mL and treated with butyrate (1 mM) alone or combined with vitamin A (10 μM) for 24 to 120 h. Cell proliferation inhibition was 34, 10 and 46% following treatment with butyrate, vitamin A and their combination, respectively, suggesting that vitamin A potentiated the inhibitory activities of butyrate. Furthermore, exposure to this short-chain fatty acid increased the level of histone H3K9 acetylation by 9.5-fold (Western blot), but not of H4K16, and increased the expression levels of p21WAF1 by 2.7-fold (Western blot) and of RARβ by 2.0-fold (quantitative real-time PCR). Our data show that RARβ may represent a molecular target for butyrate in breast cancer cells. Due to its effectiveness as a dietary HDACi, butyrate should be considered for use in combinatorial strategies with more active retinoids, especially in breast cancers in which RARβ is epigenetically altered.

Original languageEnglish (US)
Pages (from-to)841-850
Number of pages10
JournalBrazilian Journal of Medical and Biological Research
Volume45
Issue number9
DOIs
StatePublished - Sep 2012
Externally publishedYes

Keywords

  • Breast cancer
  • Butyric acid
  • Epigenetics
  • MCF-7 cells
  • Vitamin A

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