Efficacy of Ablative High-Dose-per-Fraction Radiation for Implanted Human Renal Cell Cancer in a Nude Mouse Model

Lance Walsh, Jennifer L. Stanfield, L. Chinsoo Cho, Cheng hui Chang, Kenneth Forster, Wareef Kabbani, Jeffrey A. Cadeddu, Jer Tsong Hsieh, Hak Choy, Robert Timmerman, Yair Lotan

Research output: Contribution to journalArticlepeer-review

47 Scopus citations

Abstract

Objectives: Stereotactic body radiation therapy (SBRT) is a new therapeutic paradigm that uses a very large dose per fraction treatments (ablative hypofractionation). We investigated the use of ablative hypofractionation in treating human renal cell carcinoma using a nude mouse model. Methods: Nude mice were injected subcutaneously with A498 human renal carcinoma cells. Tumour-bearing animals were radiated with three fractions (one per week) for a total dose of 48 Gy (n = 12), while untreated animals served as controls (n = 7). The mice were weighed, and tumour volumes were measured at baseline and weekly until 7 weeks post-treatment. Results: Control animals demonstrated progressive tumour growth and were sacrificed because of either tumour size or ulceration. Tumours in the treatment group grew to three times their initial size over the 3 weeks of treatment but subsequently decreased progressively to less than 30% of their initial volume. All treated tumours exhibited marked cytologic changes. Tumours from mice sacrificed before post-treatment week 4 had a mitotic count of 1-4/10 hpf. Tumours from mice sacrificed more than 4 weeks post-treatment (n = 4) demonstrated no mitoses. Conclusions: Treatment with high-dose-per-fraction radiation to 48 Gy resulted in a sustained decrease in tumour volume and marked cytologic changes. These results are preliminary-but promising-and encourage further research into this application of ablative hypofractionated radiation for kidney cancer.

Original languageEnglish (US)
Pages (from-to)795-800
Number of pages6
JournalEuropean Urology
Volume50
Issue number4
DOIs
StatePublished - Oct 2006

Keywords

  • Ablation
  • Cyberknife
  • Dose response
  • Hypofractionated radiation
  • Murine model
  • Renal cell carcinoma
  • Stereotactic body radiation therapy

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