Efficacy and Immunogenicity of Recombinant Pichinde Virus-Vectored Turkey Arthritis Reovirus Subunit Vaccine

Research output: Contribution to journalArticlepeer-review

Abstract

We created a recombinant live pichinde virus-vectored bivalent codon optimized subunit vaccine that expresses immunogenic Sigma C and Sigma B proteins of turkey arthritis reovirus. The vaccine virus could be transmitted horizontally immunizing the non-vaccinated pen mates. The vaccine was tested for efficacy against homologous (TARV SKM121) and heterologous (TARV O’Neil) virus challenge. Immunized poults produced serum neutralizing antibodies capable of neutralizing both viruses. The vaccinated and control birds showed similar body weights indicating no adverse effect on feed efficiency. Comparison of virus gene copy numbers in intestine and histologic lesion scores in tendons of vaccinated and non-vaccinated birds showed a decrease in the replication of challenge viruses in the intestine and tendons of vaccinated birds. These results indicate the potential usefulness of this vaccine.

Original languageEnglish (US)
Article number486
JournalVaccines
Volume10
Issue number4
DOIs
StatePublished - Apr 2022

Bibliographical note

Funding Information:
Acknowledgments: R.K. acknowledges the financial assistance of the Indian Council of Agricultural Research (ICAR) through the ICAR International Fellowship for PhD.

Funding Information:
Funding: This study was supported in part by a grant from Minnesota Agricultural Experiment Station’s Rapid Agricultural Response Funds (RARF).

Publisher Copyright:
© 2022 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Turkey arthritis reovirus
  • histologic lesion scores
  • live virus vaccine
  • recombinant vaccine
  • serum neutralizing antibodies
  • subunit vaccine

PubMed: MeSH publication types

  • Journal Article

Fingerprint

Dive into the research topics of 'Efficacy and Immunogenicity of Recombinant Pichinde Virus-Vectored Turkey Arthritis Reovirus Subunit Vaccine'. Together they form a unique fingerprint.

Cite this