Effects of yuja peel extract and its flavanones on osteopenia in ovariectomized rats and osteoblast differentiation

Eun Joo Jeon; Da Hye Lee; Yang Ji Kim; Jiyun Ahn; Min Jung Kim; Jin Taek Hwang; Jinyoung Hur; Mina Kim; Young Jin Jang; Tae Youl Ha; Dong Hyun Seo; Jong Suk Lee; Mi Jeong Sung; Chang Hwa Jung

doi:10.1002/mnfr.201600257

Effects of yuja peel extract and its flavanones on osteopenia in ovariectomized rats and osteoblast differentiation

Eun Joo Jeon, Da Hye Lee, Yang Ji Kim, Jiyun Ahn, Min Jung Kim, Jin Taek Hwang, Jinyoung Hur, Mina Kim, Young Jin Jang, Tae Youl Ha, Dong Hyun Seo, Jong Suk Lee, Mi Jeong Sung, Chang Hwa Jung

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

Scope: Yuja (Citrus junos Tanaka) possesses various health benefits, but its effects on bone health are unknown. In this study, the preventative effects of yuja peel ethanol extract (YPEE) on osteopenia were determined in ovariectomized (OVX) rats, and the mechanisms by which YPEE and its flavanones regulate osteoblastogenesis were examined in vitro. Methods and results: The effects of YPEE on osteoblastogenesis were investigated in MC3T3-E1 cells. YPEE promoted alkaline phosphatase (ALP) activity, mineralization, and the expression of osteoblast differentiation marker genes, such as ALP, runt-related transcription factor 2 (Runx2), and osteocalcin. YPEE and its flavanones promoted osteoblast differentiation via BMP-2-mediated p38 and the Smad1/5/8 signaling pathway. YPEE supplementation significantly decreased body weight and increased uterine weight and bone mineral density in OVX rats. Based on a micro-CT analysis of femurs, YPEE significantly attenuated osteopenia and increased trabecular volume fraction, trabecular separation, and trabecular number (p < 0.05). Conclusion: Dietary YPEE has a protective effect on OVX-induced osteopenia. YPEE and its flavanones promote osteoblastogenesis via the activation of the BMP/p38/Smad/Runx2 pathways. These results extend our knowledge of the beneficial effects of YPEE and provide a basis for the development of novel therapies for osteoporosis.

Original language	English (US)
Pages (from-to)	2587-2601
Number of pages	15
Journal	Molecular Nutrition and Food Research
Volume	60
Issue number	12
DOIs	https://doi.org/10.1002/mnfr.201600257
State	Published - Dec 1 2016
Externally published	Yes

Bibliographical note

Publisher Copyright:
© 2016 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

Flavanones
Osteoblastogenesis
Osteopenia
Ovariectomized
Yuja (Citrus junos Tanaka)

Publisher link

10.1002/mnfr.201600257

Find It

Find It at U of M Libraries

Cite this

Jeon, E. J., Lee, D. H., Kim, Y. J., Ahn, J., Kim, M. J., Hwang, J. T., Hur, J., Kim, M., Jang, Y. J., Ha, T. Y., Seo, D. H., Lee, J. S., Sung, M. J., & Jung, C. H. (2016). Effects of yuja peel extract and its flavanones on osteopenia in ovariectomized rats and osteoblast differentiation. Molecular Nutrition and Food Research, 60(12), 2587-2601. https://doi.org/10.1002/mnfr.201600257

@article{03ce760daa00454e8000a77cd863c054,

title = "Effects of yuja peel extract and its flavanones on osteopenia in ovariectomized rats and osteoblast differentiation",

abstract = "Scope: Yuja (Citrus junos Tanaka) possesses various health benefits, but its effects on bone health are unknown. In this study, the preventative effects of yuja peel ethanol extract (YPEE) on osteopenia were determined in ovariectomized (OVX) rats, and the mechanisms by which YPEE and its flavanones regulate osteoblastogenesis were examined in vitro. Methods and results: The effects of YPEE on osteoblastogenesis were investigated in MC3T3-E1 cells. YPEE promoted alkaline phosphatase (ALP) activity, mineralization, and the expression of osteoblast differentiation marker genes, such as ALP, runt-related transcription factor 2 (Runx2), and osteocalcin. YPEE and its flavanones promoted osteoblast differentiation via BMP-2-mediated p38 and the Smad1/5/8 signaling pathway. YPEE supplementation significantly decreased body weight and increased uterine weight and bone mineral density in OVX rats. Based on a micro-CT analysis of femurs, YPEE significantly attenuated osteopenia and increased trabecular volume fraction, trabecular separation, and trabecular number (p < 0.05). Conclusion: Dietary YPEE has a protective effect on OVX-induced osteopenia. YPEE and its flavanones promote osteoblastogenesis via the activation of the BMP/p38/Smad/Runx2 pathways. These results extend our knowledge of the beneficial effects of YPEE and provide a basis for the development of novel therapies for osteoporosis.",

keywords = "Flavanones, Osteoblastogenesis, Osteopenia, Ovariectomized, Yuja (Citrus junos Tanaka)",

author = "Jeon, \{Eun Joo\} and Lee, \{Da Hye\} and Kim, \{Yang Ji\} and Jiyun Ahn and Kim, \{Min Jung\} and Hwang, \{Jin Taek\} and Jinyoung Hur and Mina Kim and Jang, \{Young Jin\} and Ha, \{Tae Youl\} and Seo, \{Dong Hyun\} and Lee, \{Jong Suk\} and Sung, \{Mi Jeong\} and Jung, \{Chang Hwa\}",

note = "Publisher Copyright: {\textcopyright} 2016 WILEY-VCH Verlag GmbH \& Co. KGaA, Weinheim",

year = "2016",

month = dec,

day = "1",

doi = "10.1002/mnfr.201600257",

language = "English (US)",

volume = "60",

pages = "2587--2601",

journal = "Molecular Nutrition and Food Research",

issn = "1613-4125",

publisher = "Wiley-VCH Verlag",

number = "12",

}

TY - JOUR

T1 - Effects of yuja peel extract and its flavanones on osteopenia in ovariectomized rats and osteoblast differentiation

AU - Jeon, Eun Joo

AU - Lee, Da Hye

AU - Kim, Yang Ji

AU - Ahn, Jiyun

AU - Kim, Min Jung

AU - Hwang, Jin Taek

AU - Hur, Jinyoung

AU - Kim, Mina

AU - Jang, Young Jin

AU - Ha, Tae Youl

AU - Seo, Dong Hyun

AU - Lee, Jong Suk

AU - Sung, Mi Jeong

AU - Jung, Chang Hwa

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Scope: Yuja (Citrus junos Tanaka) possesses various health benefits, but its effects on bone health are unknown. In this study, the preventative effects of yuja peel ethanol extract (YPEE) on osteopenia were determined in ovariectomized (OVX) rats, and the mechanisms by which YPEE and its flavanones regulate osteoblastogenesis were examined in vitro. Methods and results: The effects of YPEE on osteoblastogenesis were investigated in MC3T3-E1 cells. YPEE promoted alkaline phosphatase (ALP) activity, mineralization, and the expression of osteoblast differentiation marker genes, such as ALP, runt-related transcription factor 2 (Runx2), and osteocalcin. YPEE and its flavanones promoted osteoblast differentiation via BMP-2-mediated p38 and the Smad1/5/8 signaling pathway. YPEE supplementation significantly decreased body weight and increased uterine weight and bone mineral density in OVX rats. Based on a micro-CT analysis of femurs, YPEE significantly attenuated osteopenia and increased trabecular volume fraction, trabecular separation, and trabecular number (p < 0.05). Conclusion: Dietary YPEE has a protective effect on OVX-induced osteopenia. YPEE and its flavanones promote osteoblastogenesis via the activation of the BMP/p38/Smad/Runx2 pathways. These results extend our knowledge of the beneficial effects of YPEE and provide a basis for the development of novel therapies for osteoporosis.

AB - Scope: Yuja (Citrus junos Tanaka) possesses various health benefits, but its effects on bone health are unknown. In this study, the preventative effects of yuja peel ethanol extract (YPEE) on osteopenia were determined in ovariectomized (OVX) rats, and the mechanisms by which YPEE and its flavanones regulate osteoblastogenesis were examined in vitro. Methods and results: The effects of YPEE on osteoblastogenesis were investigated in MC3T3-E1 cells. YPEE promoted alkaline phosphatase (ALP) activity, mineralization, and the expression of osteoblast differentiation marker genes, such as ALP, runt-related transcription factor 2 (Runx2), and osteocalcin. YPEE and its flavanones promoted osteoblast differentiation via BMP-2-mediated p38 and the Smad1/5/8 signaling pathway. YPEE supplementation significantly decreased body weight and increased uterine weight and bone mineral density in OVX rats. Based on a micro-CT analysis of femurs, YPEE significantly attenuated osteopenia and increased trabecular volume fraction, trabecular separation, and trabecular number (p < 0.05). Conclusion: Dietary YPEE has a protective effect on OVX-induced osteopenia. YPEE and its flavanones promote osteoblastogenesis via the activation of the BMP/p38/Smad/Runx2 pathways. These results extend our knowledge of the beneficial effects of YPEE and provide a basis for the development of novel therapies for osteoporosis.

KW - Flavanones

KW - Osteoblastogenesis

KW - Osteopenia

KW - Ovariectomized

KW - Yuja (Citrus junos Tanaka)

UR - https://www.scopus.com/pages/publications/84988396186

UR - https://www.scopus.com/inward/citedby.url?scp=84988396186&partnerID=8YFLogxK

U2 - 10.1002/mnfr.201600257

DO - 10.1002/mnfr.201600257

M3 - Article

C2 - 27506630

AN - SCOPUS:84988396186

SN - 1613-4125

VL - 60

SP - 2587

EP - 2601

JO - Molecular Nutrition and Food Research

JF - Molecular Nutrition and Food Research

IS - 12

ER -

Effects of yuja peel extract and its flavanones on osteopenia in ovariectomized rats and osteoblast differentiation

Abstract

Bibliographical note

Keywords

Publisher link

Find It

Other files and links

Fingerprint

Cite this