TY - JOUR
T1 - Effects of weekly regrouping of prepartum dairy cows on innate immune response and antibody concentration
AU - Silva, P. R.B.
AU - Moraes, J. G.N.
AU - Mendonça, L. G.D.
AU - Scanavez, A. A.
AU - Nakagawa, G.
AU - Ballou, M. A.
AU - Walcheck, B.
AU - Haines, D.
AU - Endres, M. I.
AU - Chebel, R. C.
PY - 2013/12
Y1 - 2013/12
N2 - Objectives were to evaluate the effects of a stable prepartum grouping strategy on innate immune parameters, antibody concentration, and cortisol and haptoglobin concentrations of Jersey cows. Cows (253. ±. 3. d of gestation) were paired by gestation length and assigned randomly to the stable (all-in-all-out; AIAO) or traditional (TRD) treatment. In the AIAO treatment, groups of 44 cows were moved into a pen where they remained for 5. wk, whereas in the TRD treatment, approximately 10 cows were moved into a pen weekly to maintain stocking density (44 cows for 48 headlocks). Pens were identical in size and design and each pen received each treatment a total of 3 times (6 replicates; AIAO, n. = 259; TRD, n. = 308). A subgroup of cows (n. = 34/treatment) was selected on wk 1 of each replicate from which blood was sampled weekly from d -14 to 14 (d 0. = calving) to determine polymorphonuclear leukocyte (PMNL) phagocytosis, oxidative burst, and expression of CD18 and L-selectin, hemogram, cortisol and glucose concentrations, and haptoglobin concentration. Another subgroup of cows (n. = 40/treatment) selected on wk 1 of each replicate was treated with chicken egg ovalbumin on d -21, -7, and 7 and had blood sampled weekly from d -21 to 21 for determination of immunoglobulin G anti-ovalbumin. All cows (n. = 149) had blood sampled weekly for nonesterified fatty acid (NEFA) and β-hydroxybutyrate (BHBA) concentrations from d -21 to 21. Treatment did not affect percentage of PMNL positive for phagocytosis and oxidative burst (AIAO. = 64.3. ±. 2.9 vs. TRD. = 64.3. ±. 2.9%) and intensity of phagocytosis [AIAO. = 2,910.82. ±. 405.99 vs. TRD. = 2,981.52. ±. 406.87 geometric mean fluorescence intensity (GMFI)] and oxidative burst (AIAO. = 7,667.99. ±. 678.29 vs. TRD. = 7,742.70. ±. 682.91. GMFI). Similarly, treatment did not affect the percentage of PMNL expressing CD18 (AIAO. = 96.3. ±. 0.7 vs. TRD. = 97.8. ±. 0.7%) and L-selectin (AIAO. = 44.1. ±. 2.8 vs. TRD. = 45.1. ±. 2.8%) or the intensity of expression of CD18 (AIAO. = 3,496.2. ±. 396.5 vs. TRD. = 3,598.5. ±. 396.9. GMFI) and L-selectin (AIAO. = 949.8. ±. 22.0 vs. TRD. = 940.4. ±. 22.3. GMFI). Concentration of immunoglobulin G anti-ovalbumin was not affected by treatment (AIAO. = 0.98. ±. 0.05 vs. TRD. = 0.98. ±. 0.05 OD). The percentage of leukocytes classified as granulocyte (AIAO. = 38.9. ±. 1.5 vs. TRD 38.2. ±. 1.5%) and the granulocyte:lymphocyte ratio (AIAO. = 0.75. ±. 0.04 vs. TRD. = 0.75. ±. 0.04) were not affected by treatment. Concentrations of cortisol (AIAO. = 14.95. ±. 1.73 vs. TRD. = 18.07. ±. 1.73. ng/mL), glucose (AIAO. = 57.6. ±. 1.5 vs. TRD. = 60.0. ±. 1.5. ng/mL), and haptoglobin (AIAO. = 3.09. ±. 0.48 vs. TRD. = 3.51. ±. 0.49 OD) were not affected by treatment. According to the current experiment, a stable prepartum grouping strategy does not improve innate immune parameters or antibody concentration compared with weekly prepartum regrouping.
AB - Objectives were to evaluate the effects of a stable prepartum grouping strategy on innate immune parameters, antibody concentration, and cortisol and haptoglobin concentrations of Jersey cows. Cows (253. ±. 3. d of gestation) were paired by gestation length and assigned randomly to the stable (all-in-all-out; AIAO) or traditional (TRD) treatment. In the AIAO treatment, groups of 44 cows were moved into a pen where they remained for 5. wk, whereas in the TRD treatment, approximately 10 cows were moved into a pen weekly to maintain stocking density (44 cows for 48 headlocks). Pens were identical in size and design and each pen received each treatment a total of 3 times (6 replicates; AIAO, n. = 259; TRD, n. = 308). A subgroup of cows (n. = 34/treatment) was selected on wk 1 of each replicate from which blood was sampled weekly from d -14 to 14 (d 0. = calving) to determine polymorphonuclear leukocyte (PMNL) phagocytosis, oxidative burst, and expression of CD18 and L-selectin, hemogram, cortisol and glucose concentrations, and haptoglobin concentration. Another subgroup of cows (n. = 40/treatment) selected on wk 1 of each replicate was treated with chicken egg ovalbumin on d -21, -7, and 7 and had blood sampled weekly from d -21 to 21 for determination of immunoglobulin G anti-ovalbumin. All cows (n. = 149) had blood sampled weekly for nonesterified fatty acid (NEFA) and β-hydroxybutyrate (BHBA) concentrations from d -21 to 21. Treatment did not affect percentage of PMNL positive for phagocytosis and oxidative burst (AIAO. = 64.3. ±. 2.9 vs. TRD. = 64.3. ±. 2.9%) and intensity of phagocytosis [AIAO. = 2,910.82. ±. 405.99 vs. TRD. = 2,981.52. ±. 406.87 geometric mean fluorescence intensity (GMFI)] and oxidative burst (AIAO. = 7,667.99. ±. 678.29 vs. TRD. = 7,742.70. ±. 682.91. GMFI). Similarly, treatment did not affect the percentage of PMNL expressing CD18 (AIAO. = 96.3. ±. 0.7 vs. TRD. = 97.8. ±. 0.7%) and L-selectin (AIAO. = 44.1. ±. 2.8 vs. TRD. = 45.1. ±. 2.8%) or the intensity of expression of CD18 (AIAO. = 3,496.2. ±. 396.5 vs. TRD. = 3,598.5. ±. 396.9. GMFI) and L-selectin (AIAO. = 949.8. ±. 22.0 vs. TRD. = 940.4. ±. 22.3. GMFI). Concentration of immunoglobulin G anti-ovalbumin was not affected by treatment (AIAO. = 0.98. ±. 0.05 vs. TRD. = 0.98. ±. 0.05 OD). The percentage of leukocytes classified as granulocyte (AIAO. = 38.9. ±. 1.5 vs. TRD 38.2. ±. 1.5%) and the granulocyte:lymphocyte ratio (AIAO. = 0.75. ±. 0.04 vs. TRD. = 0.75. ±. 0.04) were not affected by treatment. Concentrations of cortisol (AIAO. = 14.95. ±. 1.73 vs. TRD. = 18.07. ±. 1.73. ng/mL), glucose (AIAO. = 57.6. ±. 1.5 vs. TRD. = 60.0. ±. 1.5. ng/mL), and haptoglobin (AIAO. = 3.09. ±. 0.48 vs. TRD. = 3.51. ±. 0.49 OD) were not affected by treatment. According to the current experiment, a stable prepartum grouping strategy does not improve innate immune parameters or antibody concentration compared with weekly prepartum regrouping.
KW - Dairy cow
KW - Immune response
KW - Regrouping
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U2 - 10.3168/jds.2013-6752
DO - 10.3168/jds.2013-6752
M3 - Article
C2 - 24140318
AN - SCOPUS:84888019469
SN - 0022-0302
VL - 96
SP - 7649
EP - 7657
JO - Journal of Dairy Science
JF - Journal of Dairy Science
IS - 12
ER -