Effects of transforming growth factor-beta 1 (TGF-β1) on in vitro mineralization of human osteoblasts on implant materials

Hai Zhang, Mansur Ahmad, Gloria Gronowicz

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79 Scopus citations


An in vitro cell-implant mineralization model system was used to study the effect of transforming growth factor-beta 1(TGF-β1) on mineralization in human osteoblast cultures. SaOs-2 and primary human osteoblast (HOB) cells were cultured on Tivanium (Ti-6Al-4V) disks. Administration of different concentrations of TGF-β1 (0.02, 0.01, 0.2, 1.0, 2.0ng/ml) to these cultures demonstrated a biphasic dose response with 0.2ng/ml TGF-β1 maximally increasing the calcium content compared to control culture. Results with SaOs-2 and HOB cultures were similar. An optimal dose of TGF-β1 (0.2ng/ml) was provided to the cultures either in one single dose or multiple doses. Continuous administration of 0.2ng/ml TGF-β1 caused 77% (SaOs-2) and 60% (HOB) increases in calcification compared to the control and 0.2ng/ml single dose groups. Single administration of the accumulative dose at 1.6ng/ml had no significant effect on the calcium content in either cell culture compared to control. Two weeks continuous administration of 0.2ng/ml TGF-β1 in both cell cultures resulted in significant increases in the expression of bone specific extracellular matrix proteins which included alkaline phosphatase, Type I collagen, and osteocalcin as measured by Northern blot analysis and RT-PCR. At 4 weeks, the mRNA level of Type I collagen was still significantly higher in the TGF-β1 treatment group compared to control. In conclusion, TGF-β1 enhances mineralization of HOB on implant materials.

Original languageEnglish (US)
Pages (from-to)2013-2020
Number of pages8
Issue number12
StatePublished - May 2003

Bibliographical note

Funding Information:
This work was supported by The Patrick and Catherine Weldon Donaghue Medical Research Foundation.

Copyright 2017 Elsevier B.V., All rights reserved.


  • Growth factor
  • Implant
  • Mineralization
  • Osteoblast


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