Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor

Jie Sheng; Hyun Woo Kim; Jonathan P. Badalamenti; Chao Zhou; Swathi Sridharakrishnan; Rosa Krajmalnik-Brown; Bruce E. Rittmann; Raveender Vannela

doi:10.1016/j.biortech.2011.09.083

Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor

Jie Sheng, Hyun Woo Kim, Jonathan P. Badalamenti, Chao Zhou, Swathi Sridharakrishnan, Rosa Krajmalnik-Brown, Bruce E. Rittmann, Raveender Vannela

Genomics Center

Research output: Contribution to journal › Article › peer-review

63 Scopus citations

Abstract

Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33. °C, a higher temperature of 44. °C and lower temperatures of 22. °C and 18. °C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30. °C, 0.62 at 44. °C, and 1.29 at 18. °C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.

Original language	English (US)
Pages (from-to)	11218-11225
Number of pages	8
Journal	Bioresource Technology
Volume	102
Issue number	24
DOIs	https://doi.org/10.1016/j.biortech.2011.09.083
State	Published - Dec 2011

Bibliographical note

Funding Information:
We gratefully thank British Petroleum (BP) , Science Foundation Arizona (SFAz) , Arizona State University , and US Department of Energy (US DoE) through ARPA-E funding for providing financial support. We acknowledge the scientific help by Wim F.J. Vermaas, Robert Roberson, and other colleagues in the School of Life Sciences, Arizona State University for providing inoculum and light microscopy. We appreciate critical help by Mark Holl, Rhett Martineau, Jeff Houkal, and Juan Vela at Center for Biosignatures Discovery and Automation (formerly known as Center for Ecogenomics), the Biodesign Institute, Arizona State University for their technical help in maintaining bench-top photobioreactor operations.

Keywords

Biomass production
Fatty acids
Lipids
Synechocystis sp. PCC6803
Temperature

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2011.09.083

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title = "Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor",

abstract = "Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33. °C, a higher temperature of 44. °C and lower temperatures of 22. °C and 18. °C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30. °C, 0.62 at 44. °C, and 1.29 at 18. °C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.",

keywords = "Biomass production, Fatty acids, Lipids, Synechocystis sp. PCC6803, Temperature",

author = "Jie Sheng and Kim, {Hyun Woo} and Badalamenti, {Jonathan P.} and Chao Zhou and Swathi Sridharakrishnan and Rosa Krajmalnik-Brown and Rittmann, {Bruce E.} and Raveender Vannela",

note = "Funding Information: We gratefully thank British Petroleum (BP) , Science Foundation Arizona (SFAz) , Arizona State University , and US Department of Energy (US DoE) through ARPA-E funding for providing financial support. We acknowledge the scientific help by Wim F.J. Vermaas, Robert Roberson, and other colleagues in the School of Life Sciences, Arizona State University for providing inoculum and light microscopy. We appreciate critical help by Mark Holl, Rhett Martineau, Jeff Houkal, and Juan Vela at Center for Biosignatures Discovery and Automation (formerly known as Center for Ecogenomics), the Biodesign Institute, Arizona State University for their technical help in maintaining bench-top photobioreactor operations. ",

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T1 - Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor

AU - Sheng, Jie

AU - Kim, Hyun Woo

AU - Badalamenti, Jonathan P.

AU - Zhou, Chao

AU - Sridharakrishnan, Swathi

AU - Krajmalnik-Brown, Rosa

AU - Rittmann, Bruce E.

AU - Vannela, Raveender

N1 - Funding Information: We gratefully thank British Petroleum (BP) , Science Foundation Arizona (SFAz) , Arizona State University , and US Department of Energy (US DoE) through ARPA-E funding for providing financial support. We acknowledge the scientific help by Wim F.J. Vermaas, Robert Roberson, and other colleagues in the School of Life Sciences, Arizona State University for providing inoculum and light microscopy. We appreciate critical help by Mark Holl, Rhett Martineau, Jeff Houkal, and Juan Vela at Center for Biosignatures Discovery and Automation (formerly known as Center for Ecogenomics), the Biodesign Institute, Arizona State University for their technical help in maintaining bench-top photobioreactor operations.

PY - 2011/12

Y1 - 2011/12

N2 - Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33. °C, a higher temperature of 44. °C and lower temperatures of 22. °C and 18. °C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30. °C, 0.62 at 44. °C, and 1.29 at 18. °C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.

AB - Synechocystis sp. PCC6803 exhibited a high degree of variation in biomass and lipid production rates in response to temperature changes in a photobioreactor. Compared with an optimal temperature of 30-33. °C, a higher temperature of 44. °C and lower temperatures of 22. °C and 18. °C severely inhibited the specific growth rate (up to a 66% decrease), biomass production rate (up to a 71% decrease), nutrient utilization rates (up to a 77% decrease), and lipid production rate (up to a 80% decrease). Temperature stress triggered changes in the relative percentage of individual fatty acids (mainly for C16:0 and C18:3), and degree of unsaturation significantly changed: 0.87 at 30. °C, 0.62 at 44. °C, and 1.29 at 18. °C. Although PCC6803 survived temperature stress and maintained its predominate position in the culture, it could not fully recover from long-term temperature stress. Thus, avoiding prolonged exposure to extreme temperature is crucial for using PCC6803 as feedstock for biofuel production.

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KW - Lipids

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ER -

Effects of temperature shifts on growth rate and lipid characteristics of Synechocystis sp. PCC6803 in a bench-top photobioreactor

Abstract

Bibliographical note

Keywords

UN SDGs

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