Effects of swimming training on three superoxide dismutase isoenzymes in mouse tissues

Chitose Nakao, Tomomi Ookawara, Takako Kizaki, Shuji Oh-Ishi, Hiromi Miyazaki, Shuhkoh Haga, Yuzo Sato, Li Li Ji, Hideki Ohno

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

The purpose of the present study was to investigate the effects of swimming training on the changes in three superoxide dismutase (SOD) isoenzymes in mice. The trained mice underwent a 6-wk swimming program (1 h/day, 5 days/wk) in water at 35-36°C. Immunoreactive extracellular SOD (EC- SOD), copper- and zinc-containing SOD (CuZn-SOD), and manganese-containing SOD (Mn-SOD) contents and their mRNA abundance were determined in serum, heart, lung, liver, kidney, and gastrocnemius muscle. EC-SOD content in liver and kidney was significantly increased with training. After training, CuZn- SOD content rose significantly only in kidney but decreased significantly in heart, lung, and liver. Mn-SOD content showed a significant increase in lung, kidney, and skeletal muscle but a significant decrease in liver. In most tissues, however, the changes in SOD isoenzyme contents were not concomitant with those in their mRNA levels. The results obtained thus suggest that, except for kidney, the responses in mouse tissues of three SOD isoenzymes (protein levels and mRNA abundance) to swimming training are different and that kidney may be one of the most sensitive organs to adapt to oxidative stress during physical training, although the mechanism remains vague.

Original languageEnglish (US)
Pages (from-to)649-654
Number of pages6
JournalJournal of applied physiology
Volume88
Issue number2
DOIs
StatePublished - Feb 2000

Keywords

  • Affinity for heparin
  • Extracellular superoxide dismutase
  • mRNA

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