Effects of supraphysiological temperatures on the plasma membrane of isolated skeletal muscle cells

J. T. Padanilam, J. C. Bischof, R. C. Lee, E. G. Cravalho, R. G. Tompkins, M. L. Yarmush, M. Toner

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations

Abstract

We have investigated the effects of supraphysiological temperatures on the integrity and the permeability of the plasma membrane of isolated skeletal muscle cells. A thermal state and fluorescent microscopy system was used to expose the cells to well-defined temperatures between 37 and 70°C. The kinetics of thermal damage was assessed on real time using the fluorescent dye calcein-AM as a membrane integrity probe and the activation energy of the thermal damage process in the plasma membrane was 32.9 kcal/ mole. The F test statistically verified the superiority of the time dependent permeability fit indicating exposure to supraphysiological temperatures results in continuous hyperpermeabilization of the plasma membrane.

Original languageEnglish (US)
Title of host publicationAdvances in Bioheat and Mass Transfer
PublisherPubl by ASME
Pages69-72
Number of pages4
ISBN (Print)079181047X
StatePublished - Dec 1 1993
EventProceedings of the 1993 ASME Winter Annual Meeting - New Orleans, LA, USA
Duration: Nov 28 1993Dec 3 1993

Publication series

NameAmerican Society of Mechanical Engineers, Heat Transfer Division, (Publication) HTD
Volume268
ISSN (Print)0272-5673

Other

OtherProceedings of the 1993 ASME Winter Annual Meeting
CityNew Orleans, LA, USA
Period11/28/9312/3/93

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