Effects of retinoic acid signaling on extraocular muscle myogenic precursor cells in vitro

Sadie L. Hebert, Krysta R. Fitzpatrick, Samantha A. McConnell, Anja Cucak, Ching Yuan, Linda K. McLoon

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

One major difference between limb and extraocular muscles (EOM) is the presence of an enriched population of Pitx2-positive myogenic precursor cells in EOM compared to limb muscle. We hypothesize that retinoic acid regulates Pitx2 expression in EOM myogenic precursor cells and that its effects would differ in leg muscle. The two muscle groups expressed differential retinoic acid receptor (RAR) and retinoid X receptor (RXR) levels. RXR co-localized with the Pitx2-positive cells but not with those expressing Pax7. EOM-derived and LEG-derived EECD34 cells were treated with vehicle, retinoic acid, the RXR agonist bexarotene, the RAR inverse agonist BMS493, or the RXR antagonist UVI 3003. In vitro, fewer EOM-derived EECD34 cells expressed desmin and fused, while more LEG-derived cells expressed desmin and fused when treated with retinoic acid compared to vehicle. Both EOM and LEG-derived EECD34 cells exposed to retinoic acid showed a higher percentage of cells expressing Pitx2 compared to vehicle, supporting the hypothesis that retinoic acid plays a role in maintaining Pitx2 expression. We hypothesize that retinoic acid signaling aids in the maintenance of large numbers of undifferentiated myogenic precursor cells in the EOM, which would be required to maintain EOM normalcy throughout a lifetime of myonuclear turnover.

Original languageEnglish (US)
Pages (from-to)101-111
Number of pages11
JournalExperimental Cell Research
Volume361
Issue number1
DOIs
StatePublished - Dec 1 2017

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Oculomotor Muscles
Tretinoin
Retinoid X Receptors
Retinoic Acid Receptors
Desmin
Muscles
Extremities
In Vitro Techniques
Leg

Keywords

  • Extraocular muscle
  • Muscle stem cells
  • Retinoic acid
  • Retinoic acid signaling

Cite this

Effects of retinoic acid signaling on extraocular muscle myogenic precursor cells in vitro. / Hebert, Sadie L.; Fitzpatrick, Krysta R.; McConnell, Samantha A.; Cucak, Anja; Yuan, Ching; McLoon, Linda K.

In: Experimental Cell Research, Vol. 361, No. 1, 01.12.2017, p. 101-111.

Research output: Contribution to journalArticle

Hebert, Sadie L. ; Fitzpatrick, Krysta R. ; McConnell, Samantha A. ; Cucak, Anja ; Yuan, Ching ; McLoon, Linda K. / Effects of retinoic acid signaling on extraocular muscle myogenic precursor cells in vitro. In: Experimental Cell Research. 2017 ; Vol. 361, No. 1. pp. 101-111.
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