Effects of phenytoin on [3H]diazepam binding in dissociated primary cortical cell culture

Dorothy W. Gallager, Pierre Mallorga, Kenneth F Swaiman, Elaine A. Neale, Phillip G. Nelson

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The effects of chronic exposure of primary dissociated cerebral cortical cells in culture to the anticonvulsant drug phenytoin have been investigated using benzodiazepine binding techniques. By separating benzodiazepine binding into pharmacologically distinct subtypes, the data indicate that clonazepam-displaceable benzodiazepine binding (associated primarily with neuronal membranes) is significantly decreased by exposure to therapeutic and toxic doses of phenytoin while R05-4864-displaceable benzodiazepine binding (associated principally with non-neuronal elements) is enhanced. The ratio of clonazepam-displaceable to R05-4864-displaceable benzodiazepine binding appears to be the most sensitive indicator for these changes.

Original languageEnglish (US)
Pages (from-to)319-330
Number of pages12
JournalBrain Research
Volume218
Issue number1-2
DOIs
StatePublished - Aug 10 1981

Keywords

  • benzodiazepine binding
  • embryonic cerebral cortical cell culture
  • neuronal versus non-neuronal binding
  • phenytoin

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