Effects of phenobarbital and phenytoin on cortical glial cells in culture

Kenneth F Swaiman, Valynda L. Machen

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Studies were undertaken to determine the effects of 7-day phenobarbital and Phenytoin exposure on 14-day-old glial cell cultures of fetal murine cortex. Biochemical markers monitored were Ro5-4684-displaceable 3H-flunitrazepam binding, 3H-β-alanine uptake, glutamine synthetase activity, and protein content Phenobarbital concentrations were 30, 60, and 120 μg/ml and Phenytoin concentrations 15, 30, 60 μg/ml. There were no discernible phase microscopic changes at any concentration of either drug. Phenobarbital produced no significant changes in the biochemical measures monitored. Exposure to phenytoin produced no biochemical changes at 15 μg/ml, but did produce significant changes at 30 and 60 μg/ml. There was an increase in Ro5-4684-displaceable 3H-flunitrazepam binding signifying increased binding or an increase in the number of binding sites and perhaps an increased population of glial cells although, the unchanged protein content suggests that the number of glial cells was not increased. There was a decrease with 30 and 60 μg/ml phenytoin of 3H-β-alanine uptake suggesting interference with normal membrane transport of this compound. The latter effect may well mirror changes in GABA uptake in glial cells in the presence of phenytoin.

Original languageEnglish (US)
Pages (from-to)242-246
Number of pages5
JournalBrain and Development
Volume13
Issue number4
DOIs
StatePublished - Jan 1 1991

Keywords

  • Antiepileptic drugs
  • benzodiazepine binding
  • glial cells
  • phenobarbital
  • phenytoin

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