Effects of nicotine on brain 1-phosphatidylinositol-4-phosphate and 1-phosphatidylinositol-3,4-bisphosphate synthesis and metabolism-possible relationship to nicotine-induced behaviors

Robert J. Hitzemann; Reiko Natsuki; Horace H. Loh

doi:10.1016/0006-2952(78)90319-2

Effects of nicotine on brain 1-phosphatidylinositol-4-phosphate and 1-phosphatidylinositol-3,4-bisphosphate synthesis and metabolism-possible relationship to nicotine-induced behaviors

Robert J. Hitzemann
, Reiko Natsuki
, Horace H. Loh

Pharmacology

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The effects of nicotine and other cholinergic drugs on the initial incorporation of ³²P_i, and [³H]glycerol into 1-phosphatidylinositol-4-phosphate(DPI)and 1-phosphatidylinsitol-3,4-bisphosphate (TPI) in the rat brain microsomal fraction were studied. Nicotine and eserine significantly decreased and mecamylamine increased [³²P]- and [³H]TPI levels. Atropine had no effect on labeled TPI levels. Dose-effect studies for nicotine revealed that as little as 0.1 mg/kg of nicotine, i.p., significantly decreased the accumulation of labeled TPI. It was concluded that pharmacologically relevant and nonconvulsive doses of nicotine can markedly affect brain polyphosphoinositide synthesis and/or metabolism; the nature of the effects is consistent with theories suggesting that the conversion of TPI to DPI will release membrane lipid bound Ca²⁺ and, thus, increase membrane excitability.

Original language	English (US)
Pages (from-to)	2519-2523
Number of pages	5
Journal	Biochemical Pharmacology
Volume	27
Issue number	21
DOIs	https://doi.org/10.1016/0006-2952(78)90319-2
State	Published - 1978

Bibliographical note

Funding Information:
Acknowledgements-Thea uthorsa cknowledgteh etech-nical assistancoef BarbaraH itzemanna nd thee ditorial and typing assistanceo f BarbaraH alperin and Kaye Welch. This study was supported in part by USPHS

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Copyright 2014 Elsevier B.V., All rights reserved.

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Effects of nicotine on brain 1-phosphatidylinositol-4-phosphate and 1-phosphatidylinositol-3,4-bisphosphate synthesis and metabolism-possible relationship to nicotine-induced behaviors. / Hitzemann, Robert J.; Natsuki, Reiko; Loh, Horace H.
In: Biochemical Pharmacology, Vol. 27, No. 21, 1978, p. 2519-2523.

Research output: Contribution to journal › Article › peer-review

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title = "Effects of nicotine on brain 1-phosphatidylinositol-4-phosphate and 1-phosphatidylinositol-3,4-bisphosphate synthesis and metabolism-possible relationship to nicotine-induced behaviors",

abstract = "The effects of nicotine and other cholinergic drugs on the initial incorporation of 32Pi, and [3H]glycerol into 1-phosphatidylinositol-4-phosphate(DPI)and 1-phosphatidylinsitol-3,4-bisphosphate (TPI) in the rat brain microsomal fraction were studied. Nicotine and eserine significantly decreased and mecamylamine increased [32P]- and [3H]TPI levels. Atropine had no effect on labeled TPI levels. Dose-effect studies for nicotine revealed that as little as 0.1 mg/kg of nicotine, i.p., significantly decreased the accumulation of labeled TPI. It was concluded that pharmacologically relevant and nonconvulsive doses of nicotine can markedly affect brain polyphosphoinositide synthesis and/or metabolism; the nature of the effects is consistent with theories suggesting that the conversion of TPI to DPI will release membrane lipid bound Ca2+ and, thus, increase membrane excitability.",

author = "Hitzemann, \{Robert J.\} and Reiko Natsuki and Loh, \{Horace H.\}",

note = "Funding Information: Acknowledgements-Thea uthorsa cknowledgteh etech-nical assistancoef BarbaraH itzemanna nd thee ditorial and typing assistanceo f BarbaraH alperin and Kaye Welch. This study was supported in part by USPHS Copyright: Copyright 2014 Elsevier B.V., All rights reserved.",

year = "1978",

doi = "10.1016/0006-2952(78)90319-2",

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pages = "2519--2523",

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AU - Hitzemann, Robert J.

AU - Natsuki, Reiko

AU - Loh, Horace H.

N1 - Funding Information: Acknowledgements-Thea uthorsa cknowledgteh etech-nical assistancoef BarbaraH itzemanna nd thee ditorial and typing assistanceo f BarbaraH alperin and Kaye Welch. This study was supported in part by USPHS Copyright: Copyright 2014 Elsevier B.V., All rights reserved.

PY - 1978

Y1 - 1978

N2 - The effects of nicotine and other cholinergic drugs on the initial incorporation of 32Pi, and [3H]glycerol into 1-phosphatidylinositol-4-phosphate(DPI)and 1-phosphatidylinsitol-3,4-bisphosphate (TPI) in the rat brain microsomal fraction were studied. Nicotine and eserine significantly decreased and mecamylamine increased [32P]- and [3H]TPI levels. Atropine had no effect on labeled TPI levels. Dose-effect studies for nicotine revealed that as little as 0.1 mg/kg of nicotine, i.p., significantly decreased the accumulation of labeled TPI. It was concluded that pharmacologically relevant and nonconvulsive doses of nicotine can markedly affect brain polyphosphoinositide synthesis and/or metabolism; the nature of the effects is consistent with theories suggesting that the conversion of TPI to DPI will release membrane lipid bound Ca2+ and, thus, increase membrane excitability.

AB - The effects of nicotine and other cholinergic drugs on the initial incorporation of 32Pi, and [3H]glycerol into 1-phosphatidylinositol-4-phosphate(DPI)and 1-phosphatidylinsitol-3,4-bisphosphate (TPI) in the rat brain microsomal fraction were studied. Nicotine and eserine significantly decreased and mecamylamine increased [32P]- and [3H]TPI levels. Atropine had no effect on labeled TPI levels. Dose-effect studies for nicotine revealed that as little as 0.1 mg/kg of nicotine, i.p., significantly decreased the accumulation of labeled TPI. It was concluded that pharmacologically relevant and nonconvulsive doses of nicotine can markedly affect brain polyphosphoinositide synthesis and/or metabolism; the nature of the effects is consistent with theories suggesting that the conversion of TPI to DPI will release membrane lipid bound Ca2+ and, thus, increase membrane excitability.

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ER -

Effects of nicotine on brain 1-phosphatidylinositol-4-phosphate and 1-phosphatidylinositol-3,4-bisphosphate synthesis and metabolism-possible relationship to nicotine-induced behaviors

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