Effects of microalgae-bacteria inoculation ratio on biogas slurry treatment and microorganism interactions in the symbiosis system

Qiaoyun Huang, Hongbin Yan, Yuhuan Liu, Xian Cui, Yunpu Wang, Zhigang Yu, Roger Ruan, Qi Zhang

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18 Scopus citations


The improper disposal of biogas slurry will result in significant pollution of the environment. Microalgae-bacteria symbiosis systems can effectively treat biogas slurry while also producing useful biomass. In this study, Chlorella vulgaris (C.V) and indigenous Shinella sp. YHB03 (S.S) were co-cultured and mono-cultured to purify biogas slurry. The inoculation ratio of C.V and S.S in the co-culture was optimized. The enhancement rate was creatively introduced to quantitatively analyze the changes between co-cultures and mono-cultures. The introduction of S.S into the C.V culture increased the carbon emission reduction capacity of the system, improved the microbe's tolerance to biogas slurry, and raised algal cell viability. Biomass production, pollutants removal and value-added products output were significantly enhanced when co-cultured C.V with S.S. And as the inoculation ratio of C.V and S.S increased from 2:1 to 50:1, this synergistic effect increased at first, then dropped, with 20:1 being the optimal. When co-cultured C.V with S.S at inoculation ratio of 20:1, the microalgae cell viability attained 84.84%, the biomass productivity reached 80 mg·L−1·day−1, and the NH4+-N, TP, TOC and IC removal efficiencies achieved 72.2%, 48.3%, 65% and 63.4%, respectively. This study provides an environmentally sustainable strategy for biogas slurry treatment.

Original languageEnglish (US)
Article number132271
JournalJournal of Cleaner Production
StatePublished - Aug 15 2022

Bibliographical note

Funding Information:
This work was supported by the National Natural Science Foundation of China (No. 22106062 , 21878139 , 21878237 , and 22166026 ) and the Natural Science Foundation of Jiangxi Province (No. 20212BAB214063 , 20181BBF60026 ).

Publisher Copyright:
© 2022 Elsevier Ltd


  • Biomass production
  • Co-culture
  • Enhancement rate
  • Nutrient removal
  • Pollutant tolerance


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