Effects of methylene blue photodynamic therapy on oral carcinoma and leukoplakia cells

Mina N. Le; Beverly R Wuertz; Merrill A. Biel; Rachel L. Thompson; Frank G. Ondrey

doi:10.1002/lio2.772

Effects of methylene blue photodynamic therapy on oral carcinoma and leukoplakia cells

Mina N. Le, Beverly R Wuertz, Merrill A. Biel, Rachel L. Thompson, Frank G. Ondrey

Otolaryngology

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: Methylene blue (MB) is a readily available and affordable substrate that can be used as a photosensitizer for photodynamic therapy (PDT). The objective of this study was to determine if PDT with MB can downregulate matrix metalloproteinases (MMPs) related to oral carcinoma. Methods: Cell cultures of oral squamous cell carcinoma (CA-9-22), oral leukoplakia (MSK-Leuk1), and immortalized keratinocytes (Rhek-1A) were photosensitized with MB and treated with PDT. MMP-9 gene expression was interrogated via qRT-PCR. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to confirm the efficacy of MB PDT. Results: MMP-9 gene expression was found to be significantly decreased in oral carcinoma, leukoplakia, and immortalized keratinocytes with use of MB PDT. Conclusion: This work demonstrates that MB-mediated PDT can downregulate MMPs which are critical to the invasion and metastasis of oral cancer. These results suggest that MB PDT could be a clinically significant and cost-effective treatment for oral leukoplakia and carcinoma. Level of Evidence: NA.

Original language	English (US)
Pages (from-to)	982-987
Number of pages	6
Journal	Laryngoscope investigative otolaryngology
Volume	7
Issue number	4
DOIs	https://doi.org/10.1002/lio2.772
State	Published - Aug 2022

Bibliographical note

Funding Information:
The authors wish to acknowledge the Lions International Medical Research Foundation who provided funding for this project through the Lions 5M Club of Minnesota.

Publisher Copyright:
© 2022 The Authors. Laryngoscope Investigative Otolaryngology published by Wiley Periodicals LLC on behalf of The Triological Society.

Keywords

molecular biology
oral cavity
oropharynx

PubMed: MeSH publication types

Journal Article

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10.1002/lio2.772

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title = "Effects of methylene blue photodynamic therapy on oral carcinoma and leukoplakia cells",

abstract = "Objective: Methylene blue (MB) is a readily available and affordable substrate that can be used as a photosensitizer for photodynamic therapy (PDT). The objective of this study was to determine if PDT with MB can downregulate matrix metalloproteinases (MMPs) related to oral carcinoma. Methods: Cell cultures of oral squamous cell carcinoma (CA-9-22), oral leukoplakia (MSK-Leuk1), and immortalized keratinocytes (Rhek-1A) were photosensitized with MB and treated with PDT. MMP-9 gene expression was interrogated via qRT-PCR. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to confirm the efficacy of MB PDT. Results: MMP-9 gene expression was found to be significantly decreased in oral carcinoma, leukoplakia, and immortalized keratinocytes with use of MB PDT. Conclusion: This work demonstrates that MB-mediated PDT can downregulate MMPs which are critical to the invasion and metastasis of oral cancer. These results suggest that MB PDT could be a clinically significant and cost-effective treatment for oral leukoplakia and carcinoma. Level of Evidence: NA.",

keywords = "molecular biology, oral cavity, oropharynx",

author = "Le, {Mina N.} and Wuertz, {Beverly R} and Biel, {Merrill A.} and Thompson, {Rachel L.} and Ondrey, {Frank G.}",

note = "Funding Information: The authors wish to acknowledge the Lions International Medical Research Foundation who provided funding for this project through the Lions 5M Club of Minnesota. Publisher Copyright: {\textcopyright} 2022 The Authors. Laryngoscope Investigative Otolaryngology published by Wiley Periodicals LLC on behalf of The Triological Society.",

year = "2022",

month = aug,

doi = "10.1002/lio2.772",

language = "English (US)",

volume = "7",

pages = "982--987",

journal = "Laryngoscope investigative otolaryngology",

issn = "2378-8039",

publisher = "Wiley-Blackwell",

number = "4",

}

TY - JOUR

T1 - Effects of methylene blue photodynamic therapy on oral carcinoma and leukoplakia cells

AU - Le, Mina N.

AU - Wuertz, Beverly R

AU - Biel, Merrill A.

AU - Thompson, Rachel L.

AU - Ondrey, Frank G.

N1 - Funding Information: The authors wish to acknowledge the Lions International Medical Research Foundation who provided funding for this project through the Lions 5M Club of Minnesota. Publisher Copyright: © 2022 The Authors. Laryngoscope Investigative Otolaryngology published by Wiley Periodicals LLC on behalf of The Triological Society.

PY - 2022/8

Y1 - 2022/8

N2 - Objective: Methylene blue (MB) is a readily available and affordable substrate that can be used as a photosensitizer for photodynamic therapy (PDT). The objective of this study was to determine if PDT with MB can downregulate matrix metalloproteinases (MMPs) related to oral carcinoma. Methods: Cell cultures of oral squamous cell carcinoma (CA-9-22), oral leukoplakia (MSK-Leuk1), and immortalized keratinocytes (Rhek-1A) were photosensitized with MB and treated with PDT. MMP-9 gene expression was interrogated via qRT-PCR. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to confirm the efficacy of MB PDT. Results: MMP-9 gene expression was found to be significantly decreased in oral carcinoma, leukoplakia, and immortalized keratinocytes with use of MB PDT. Conclusion: This work demonstrates that MB-mediated PDT can downregulate MMPs which are critical to the invasion and metastasis of oral cancer. These results suggest that MB PDT could be a clinically significant and cost-effective treatment for oral leukoplakia and carcinoma. Level of Evidence: NA.

AB - Objective: Methylene blue (MB) is a readily available and affordable substrate that can be used as a photosensitizer for photodynamic therapy (PDT). The objective of this study was to determine if PDT with MB can downregulate matrix metalloproteinases (MMPs) related to oral carcinoma. Methods: Cell cultures of oral squamous cell carcinoma (CA-9-22), oral leukoplakia (MSK-Leuk1), and immortalized keratinocytes (Rhek-1A) were photosensitized with MB and treated with PDT. MMP-9 gene expression was interrogated via qRT-PCR. The 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used to confirm the efficacy of MB PDT. Results: MMP-9 gene expression was found to be significantly decreased in oral carcinoma, leukoplakia, and immortalized keratinocytes with use of MB PDT. Conclusion: This work demonstrates that MB-mediated PDT can downregulate MMPs which are critical to the invasion and metastasis of oral cancer. These results suggest that MB PDT could be a clinically significant and cost-effective treatment for oral leukoplakia and carcinoma. Level of Evidence: NA.

KW - molecular biology

KW - oral cavity

KW - oropharynx

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ER -

Effects of methylene blue photodynamic therapy on oral carcinoma and leukoplakia cells

Abstract

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