Effects of intracerebroventricular injection of neuropeptide Y on energy metabolism

C. J. Billington, J. E. Briggs, M. Grace, A. S. Levine

Research output: Contribution to journalArticlepeer-review

415 Scopus citations

Abstract

Our objective was to find out if central injection of neuropeptide Y (NPY) would alter brown fat thermogenesis and white fat lipoprotein lipase activity. The following three groups of Sprague-Dawley rats received five injections over 24 h into the right lateral ventricle: 1) NPY (5 μg/injection) and ad libitum food; 2) NPY (5 μg/injection) and food restricted to control intake; 3) saline injection and ad libitum food. The NPY ad libitum-fed group consumed more food than the saline controls or NPY food-restricted animals. Brown fat thermogenic activity, assessed by GDP binding, was decreased relative to saline controls in both NPY-treated groups. White fat lipoprotein lipase activity was greatly increased in both NPY treatment groups compared with saline controls. The NPY effects on brown and white fat were not explained by measures of serum insulin, glucagon, glucose, or other metabolites. In a follow-up experiment, we asked whether food was necessary for expression of the NPY effects. Brown fat mitochondrial GDP binding indicated NPY effect even when no food was ingested. We conclude that intracerebroventricular administration of NPY promotes white fat lipid storage and decreases brown fat thermogenesis in addition to its known effect of stimulating food intake.

Original languageEnglish (US)
Pages (from-to)R321-R327
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume260
Issue number2 29-2
DOIs
StatePublished - 1991

Keywords

  • Adipose tissue
  • Brown adipose tissue
  • Lipoprotein lipase

Fingerprint Dive into the research topics of 'Effects of intracerebroventricular injection of neuropeptide Y on energy metabolism'. Together they form a unique fingerprint.

Cite this