Effects of intense pulsed light and gamma irradiation on Bacillus cereus spores in mesquite pod flour

Dongjie Chen; Wes Mosher; Justin Wiertzema; Peng Peng; Min Min; Yanling Cheng; Jun An; Yiwei Ma; Xuetong Fan; Brendan A. Niemira; David J. Baumler; Chi Chen; Paul Chen; Roger Ruan Chen

doi:10.1016/j.foodchem.2020.128675

Effects of intense pulsed light and gamma irradiation on Bacillus cereus spores in mesquite pod flour

Dongjie Chen, Wes Mosher, Justin Wiertzema, Peng Peng, Min Min, Yanling Cheng, Jun An, Yiwei Ma, Xuetong Fan, Brendan A. Niemira, David J. Baumler, Chi Chen, Paul Chen, Roger Ruan Chen

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Abstract

This study was conducted to evaluate the inactivation of Bacillus cereus spore in mesquite flour with intense pulsed light (IPL) and gamma radiation. The physical, chemical, and toxicity of treated mesquite flour were also investigated. The results showed that up to 3.51 log₁₀CFU/g B. cereus spore inactivation was achieved with 8 kGy of gamma radiation, and up to 1.69 log₁₀CFU/g reductions could be achieved after 28s of catalytic IPL exposure. Although chemometric analysis showed 9-hydroxy-10,12-octadecadienoic acid was slightly increased after a 28s-catalytic IPL treatment, the concentration is within the acceptable range. No significant increase in acetic or propionic acids (typical off-flavor volatile compounds) was observed after either treatment. For cytotoxicity, the Caco-2 cell viability analysis revealed that these two technologies did not induce significant cytotoxicity to the treated mesquite flour. Overall, these two technologies exhibit strong potential for the decontamination of B. cereus in mesquite flour.

Original language	English (US)
Article number	128675
Journal	Food Chemistry
Volume	344
DOIs	https://doi.org/10.1016/j.foodchem.2020.128675
State	Published - May 15 2021

Bibliographical note

Funding Information:
This work is supported by the Enhancing Food Safety through Improved Processing Technologies Program [grant no. 2016-68003-24850] from the USDA National Institute of Food and Agriculture.

Funding Information:
General: We thank all participate from the Department of Bioproducts and Biosystems Engineering and Department of Food Science and Nutrition, University of Minnesota. This work is supported by the Enhancing Food Safety through Improved Processing Technologies Program [grant no. 2016-68003-24850] from the USDA National Institute of Food and Agriculture. D. C. and X. F. conceived and designed the research. W. M. performed the LC-MS based chemometric analysis. J. R. W. conducted B. cereus enumeration. B. N. accomplished the gamma radiation treatment. D. C. carried out system development, IPL treatment, and work with J. A. to conduct cell viability preparation and analysis. D. C. drafted the manuscript, W. M. J. R. W. P. C. and R. R. made critical revisions for the manuscript. P. P. M. M. Y. C. D. B. P. C. C. C, and R. R. provided important instructions of the prototype IPL system development. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. All data needed to evaluate the conclusions in the paper are present in the paper. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). The USDA is an equal opportunity provider and employer.

Publisher Copyright:
© 2020 Elsevier Ltd

Keywords

Bacillus cereus spores
Gamma radiation
Intense pulsed light
Mesquite flour

PubMed: MeSH publication types

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10.1016/j.foodchem.2020.128675

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title = "Effects of intense pulsed light and gamma irradiation on Bacillus cereus spores in mesquite pod flour",

abstract = "This study was conducted to evaluate the inactivation of Bacillus cereus spore in mesquite flour with intense pulsed light (IPL) and gamma radiation. The physical, chemical, and toxicity of treated mesquite flour were also investigated. The results showed that up to 3.51 log10CFU/g B. cereus spore inactivation was achieved with 8 kGy of gamma radiation, and up to 1.69 log10CFU/g reductions could be achieved after 28s of catalytic IPL exposure. Although chemometric analysis showed 9-hydroxy-10,12-octadecadienoic acid was slightly increased after a 28s-catalytic IPL treatment, the concentration is within the acceptable range. No significant increase in acetic or propionic acids (typical off-flavor volatile compounds) was observed after either treatment. For cytotoxicity, the Caco-2 cell viability analysis revealed that these two technologies did not induce significant cytotoxicity to the treated mesquite flour. Overall, these two technologies exhibit strong potential for the decontamination of B. cereus in mesquite flour.",

keywords = "Bacillus cereus spores, Gamma radiation, Intense pulsed light, Mesquite flour",

author = "Dongjie Chen and Wes Mosher and Justin Wiertzema and Peng Peng and Min Min and Yanling Cheng and Jun An and Yiwei Ma and Xuetong Fan and Niemira, {Brendan A.} and Baumler, {David J.} and Chi Chen and Paul Chen and {Ruan Chen}, Roger",

note = "Funding Information: This work is supported by the Enhancing Food Safety through Improved Processing Technologies Program [grant no. 2016-68003-24850] from the USDA National Institute of Food and Agriculture. Funding Information: General: We thank all participate from the Department of Bioproducts and Biosystems Engineering and Department of Food Science and Nutrition, University of Minnesota. This work is supported by the Enhancing Food Safety through Improved Processing Technologies Program [grant no. 2016-68003-24850] from the USDA National Institute of Food and Agriculture. D. C. and X. F. conceived and designed the research. W. M. performed the LC-MS based chemometric analysis. J. R. W. conducted B. cereus enumeration. B. N. accomplished the gamma radiation treatment. D. C. carried out system development, IPL treatment, and work with J. A. to conduct cell viability preparation and analysis. D. C. drafted the manuscript, W. M. J. R. W. P. C. and R. R. made critical revisions for the manuscript. P. P. M. M. Y. C. D. B. P. C. C. C, and R. R. provided important instructions of the prototype IPL system development. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. All data needed to evaluate the conclusions in the paper are present in the paper. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). The USDA is an equal opportunity provider and employer. Publisher Copyright: {\textcopyright} 2020 Elsevier Ltd",

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doi = "10.1016/j.foodchem.2020.128675",

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T1 - Effects of intense pulsed light and gamma irradiation on Bacillus cereus spores in mesquite pod flour

AU - Chen, Dongjie

AU - Mosher, Wes

AU - Wiertzema, Justin

AU - Peng, Peng

AU - Min, Min

AU - Cheng, Yanling

AU - An, Jun

AU - Ma, Yiwei

AU - Fan, Xuetong

AU - Niemira, Brendan A.

AU - Baumler, David J.

AU - Chen, Chi

AU - Chen, Paul

AU - Ruan Chen, Roger

N1 - Funding Information: This work is supported by the Enhancing Food Safety through Improved Processing Technologies Program [grant no. 2016-68003-24850] from the USDA National Institute of Food and Agriculture. Funding Information: General: We thank all participate from the Department of Bioproducts and Biosystems Engineering and Department of Food Science and Nutrition, University of Minnesota. This work is supported by the Enhancing Food Safety through Improved Processing Technologies Program [grant no. 2016-68003-24850] from the USDA National Institute of Food and Agriculture. D. C. and X. F. conceived and designed the research. W. M. performed the LC-MS based chemometric analysis. J. R. W. conducted B. cereus enumeration. B. N. accomplished the gamma radiation treatment. D. C. carried out system development, IPL treatment, and work with J. A. to conduct cell viability preparation and analysis. D. C. drafted the manuscript, W. M. J. R. W. P. C. and R. R. made critical revisions for the manuscript. P. P. M. M. Y. C. D. B. P. C. C. C, and R. R. provided important instructions of the prototype IPL system development. The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper. All data needed to evaluate the conclusions in the paper are present in the paper. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture (USDA). The USDA is an equal opportunity provider and employer. Publisher Copyright: © 2020 Elsevier Ltd

PY - 2021/5/15

Y1 - 2021/5/15

N2 - This study was conducted to evaluate the inactivation of Bacillus cereus spore in mesquite flour with intense pulsed light (IPL) and gamma radiation. The physical, chemical, and toxicity of treated mesquite flour were also investigated. The results showed that up to 3.51 log10CFU/g B. cereus spore inactivation was achieved with 8 kGy of gamma radiation, and up to 1.69 log10CFU/g reductions could be achieved after 28s of catalytic IPL exposure. Although chemometric analysis showed 9-hydroxy-10,12-octadecadienoic acid was slightly increased after a 28s-catalytic IPL treatment, the concentration is within the acceptable range. No significant increase in acetic or propionic acids (typical off-flavor volatile compounds) was observed after either treatment. For cytotoxicity, the Caco-2 cell viability analysis revealed that these two technologies did not induce significant cytotoxicity to the treated mesquite flour. Overall, these two technologies exhibit strong potential for the decontamination of B. cereus in mesquite flour.

AB - This study was conducted to evaluate the inactivation of Bacillus cereus spore in mesquite flour with intense pulsed light (IPL) and gamma radiation. The physical, chemical, and toxicity of treated mesquite flour were also investigated. The results showed that up to 3.51 log10CFU/g B. cereus spore inactivation was achieved with 8 kGy of gamma radiation, and up to 1.69 log10CFU/g reductions could be achieved after 28s of catalytic IPL exposure. Although chemometric analysis showed 9-hydroxy-10,12-octadecadienoic acid was slightly increased after a 28s-catalytic IPL treatment, the concentration is within the acceptable range. No significant increase in acetic or propionic acids (typical off-flavor volatile compounds) was observed after either treatment. For cytotoxicity, the Caco-2 cell viability analysis revealed that these two technologies did not induce significant cytotoxicity to the treated mesquite flour. Overall, these two technologies exhibit strong potential for the decontamination of B. cereus in mesquite flour.

KW - Bacillus cereus spores

KW - Gamma radiation

KW - Intense pulsed light

KW - Mesquite flour

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Effects of intense pulsed light and gamma irradiation on Bacillus cereus spores in mesquite pod flour

Abstract

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Keywords

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