Effects of human neutrophils and monocytes on Nocardia asteroides: Failure of killing despite occurrence of the oxidative metabolic burst

G. A. Filice, B. L. Beaman, J. A. Krick, J. S. Remington

Research output: Contribution to journalArticlepeer-review

30 Scopus citations

Abstract

To study mechanisms of resistance to challenged asteroides in vitro, suspensions of human neutrophils and monocytes were chanllenged with N. asteroides in the presence of autologous serum. At 4 hr, numbers of viable N. asteroides incubated with neutrophils and monocytes averaged only 10% and 21% less, respectively, than numbers of N. asteroides incubated without phagocytes. In contrast, a mean of >90% of Listeria monocytogenes and Staphylococcus aureus incubated with neutrophils and monocytes was killed. The failure to kill N. asteroides was not associated with absence of an oxidative metabolic burst by the phagocytes, since chemiluminescence occurred when phagocytes encountered N. asteroides and was blocked in preparations of N. asteroides and neutrophils by preincubation of the neutrophils with chlorpromazine. Thus, when cultured in suspension, human neutrophils and monocytes are unable to kill significant numbers of N. asteroides despite the occurrence of an oxidative metabolic burst.

Original languageEnglish (US)
Pages (from-to)432-438
Number of pages7
JournalJournal of Infectious Diseases
Volume142
Issue number3
DOIs
StatePublished - Jan 1 1980

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