Effects of gene choice, base composition and rate heterogeneity on inference and estimates of divergence times in cypriniform fishes

M. Vincent Hirt, Gloria Arratia, Wei Jen Chen, Richard L. Mayden, Kevin L. Tang, Robert M. Wood, Andrew M. Simons

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Gene choice, base compositional heterogeneity and rate heterogeneity are known to influence phylogenetic reconstruction but are often overlooked. Here, we investigate the impact of these factors in reconstructing the phylogenetic relationships and divergence times of cypriniform fishes. We compile a multilocus data set of newly sequenced and previously published nuclear protein-coding genes from species from all major lineages of Cypriniformes. We collect data on the oldest known cypriniform fossils and gather body size information. We demonstrate that body size correlates with rates of molecular evolution and contributes to rate heterogeneity not only in Cypriniformes but also in a number of other fish lineages. We find that miniaturized cypriniforms show exceptionally high rates of molecular evolution that may lead to improper phylogenetic placement of these taxa due to issues associated with branch attraction. We identify and correct for base compositional heterogeneity and find that this has a marked impact on topology and a corresponding impact on the estimation of divergence times within Cypriniformes. Using gene sequences, fossil calibrations and two different methods, we show strong evidence that Catostomidae is sister to all other cypriniform families and the major clades of cypriniform fishes diverged long before their fossil record indicates.

Original languageEnglish (US)
Pages (from-to)319-339
Number of pages21
JournalBiological Journal of the Linnean Society
Volume121
Issue number2
DOIs
StatePublished - Jun 1 2017

Bibliographical note

Funding Information:
We dedicate this study to the memory of Miles M. Coburn, a good friend, colleague and ichthyologist. MVH thanks Pamela B. Weisenhorn for help with R. We thank Jon Armbruster and one anonymous reviewer for helpful feedback. This work was supported by the Minnesota Agricultural Experiment Station and the National Science Foundation (EF 0431132 to AMS; EF 0431326 to RLM and RMW).

Publisher Copyright:
© 2017 The Linnean Society of London.

Keywords

  • Age
  • Body size
  • Catostomidae
  • Fish
  • Miniature
  • Miniaturized
  • Paedocypris
  • Phylogenetics
  • Phylogeny
  • Positive selection

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