Effects of dissolved oxygen and pH on nitrous oxide production rates in autotrophic partial nitrification granules

Rathnayake M.L.D. Rathnayake; Mamoru Oshiki; Satoshi Ishii; Takahiro Segawa; Hisashi Satoh; Satoshi Okabe

doi:10.1016/j.biortech.2015.08.054

Effects of dissolved oxygen and pH on nitrous oxide production rates in autotrophic partial nitrification granules

Rathnayake M.L.D. Rathnayake, Mamoru Oshiki, Satoshi Ishii, Takahiro Segawa, Hisashi Satoh, Satoshi Okabe

Soil, Water, and Climate

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Abstract

The effects of dissolved oxygen (DO) and pH on nitrous oxide (N₂O) production rates and pathways in autotrophic partial nitrification (PN) granules were investigated at the granular level. N₂O was primarily produced by betaproteobacterial ammonia-oxidizing bacteria, mainly Nitrosomonas europaea, in the oxic surface layer (<200μm) of the autotrophic PN granules. N₂O production increased with increasing bulk DO concentration owing to activation of the ammonia (i.e., hydroxylamine) oxidation in this layer. The highest N₂O emissions were observed at pH 7.5, although the ammonia oxidation rate was unchanged between pH 6.5 and 8.5. Overall, the results of this study suggest that in situ analyses of PN granules are essential to gaining insight into N₂O emission mechanisms in a granule.

Original language	English (US)
Pages (from-to)	15-22
Number of pages	8
Journal	Bioresource Technology
Volume	197
DOIs	https://doi.org/10.1016/j.biortech.2015.08.054
State	Published - Dec 1 2015

Bibliographical note

Funding Information:
This research was financially supported by grants from the Japan Science and Technology Agency (JST) CREST, Nagase Science and Technology Foundation, and Institute for Fermentation, Osaka (IFO) to S. Okabe. We thank Reiko Hirano and Ayumi Akiyoshi for their technical assistance.

Publisher Copyright:
© 2015 Elsevier Ltd.

Keywords

16S rRNA gene deep sequencing
Autotrophic partial nitrification granule
Dissolved oxygen effect
NO emissions
NO microsensor

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This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biortech.2015.08.054

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title = "Effects of dissolved oxygen and pH on nitrous oxide production rates in autotrophic partial nitrification granules",

abstract = "The effects of dissolved oxygen (DO) and pH on nitrous oxide (N2O) production rates and pathways in autotrophic partial nitrification (PN) granules were investigated at the granular level. N2O was primarily produced by betaproteobacterial ammonia-oxidizing bacteria, mainly Nitrosomonas europaea, in the oxic surface layer (<200μm) of the autotrophic PN granules. N2O production increased with increasing bulk DO concentration owing to activation of the ammonia (i.e., hydroxylamine) oxidation in this layer. The highest N2O emissions were observed at pH 7.5, although the ammonia oxidation rate was unchanged between pH 6.5 and 8.5. Overall, the results of this study suggest that in situ analyses of PN granules are essential to gaining insight into N2O emission mechanisms in a granule.",

keywords = "16S rRNA gene deep sequencing, Autotrophic partial nitrification granule, Dissolved oxygen effect, NO emissions, NO microsensor",

author = "Rathnayake, {Rathnayake M.L.D.} and Mamoru Oshiki and Satoshi Ishii and Takahiro Segawa and Hisashi Satoh and Satoshi Okabe",

note = "Funding Information: This research was financially supported by grants from the Japan Science and Technology Agency (JST) CREST, Nagase Science and Technology Foundation, and Institute for Fermentation, Osaka (IFO) to S. Okabe. We thank Reiko Hirano and Ayumi Akiyoshi for their technical assistance. Publisher Copyright: {\textcopyright} 2015 Elsevier Ltd.",

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doi = "10.1016/j.biortech.2015.08.054",

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AU - Rathnayake, Rathnayake M.L.D.

AU - Oshiki, Mamoru

AU - Ishii, Satoshi

AU - Segawa, Takahiro

AU - Satoh, Hisashi

AU - Okabe, Satoshi

N1 - Funding Information: This research was financially supported by grants from the Japan Science and Technology Agency (JST) CREST, Nagase Science and Technology Foundation, and Institute for Fermentation, Osaka (IFO) to S. Okabe. We thank Reiko Hirano and Ayumi Akiyoshi for their technical assistance. Publisher Copyright: © 2015 Elsevier Ltd.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - The effects of dissolved oxygen (DO) and pH on nitrous oxide (N2O) production rates and pathways in autotrophic partial nitrification (PN) granules were investigated at the granular level. N2O was primarily produced by betaproteobacterial ammonia-oxidizing bacteria, mainly Nitrosomonas europaea, in the oxic surface layer (<200μm) of the autotrophic PN granules. N2O production increased with increasing bulk DO concentration owing to activation of the ammonia (i.e., hydroxylamine) oxidation in this layer. The highest N2O emissions were observed at pH 7.5, although the ammonia oxidation rate was unchanged between pH 6.5 and 8.5. Overall, the results of this study suggest that in situ analyses of PN granules are essential to gaining insight into N2O emission mechanisms in a granule.

AB - The effects of dissolved oxygen (DO) and pH on nitrous oxide (N2O) production rates and pathways in autotrophic partial nitrification (PN) granules were investigated at the granular level. N2O was primarily produced by betaproteobacterial ammonia-oxidizing bacteria, mainly Nitrosomonas europaea, in the oxic surface layer (<200μm) of the autotrophic PN granules. N2O production increased with increasing bulk DO concentration owing to activation of the ammonia (i.e., hydroxylamine) oxidation in this layer. The highest N2O emissions were observed at pH 7.5, although the ammonia oxidation rate was unchanged between pH 6.5 and 8.5. Overall, the results of this study suggest that in situ analyses of PN granules are essential to gaining insight into N2O emission mechanisms in a granule.

KW - 16S rRNA gene deep sequencing

KW - Autotrophic partial nitrification granule

KW - Dissolved oxygen effect

KW - NO emissions

KW - NO microsensor

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JO - Agricultural Wastes

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ER -

Effects of dissolved oxygen and pH on nitrous oxide production rates in autotrophic partial nitrification granules

Abstract

Bibliographical note

Keywords

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