Effects of Dentin Debris on the Antimicrobial Properties of Sodium Hypochlorite and Etidronic Acid

Maria Teresa Arias-Moliz, Ana Morago, Ronald Ordinola-Zapata, Carmen María Ferrer-Luque, Matilde Ruiz-Linares, Pilar Baca

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34 Scopus citations


Introduction The purpose of this study was to determine the influence of dentin powder on the concentration, pH, and antimicrobial activity of sodium hypochlorite (NaOCl) alone and combined with etidronic acid (HEBP). Methods Biofilms of Enterococcus faecalis were grown on the surface of dentin blocks for 5 days and then exposed to 1% and 2.5% NaOCl alone or combined with 9% HEBP for 3 minutes in the absence and presence of dentin powder. The biovolumes of the biofilm were measured using confocal microscopy and the live/dead technique. The available chlorine and pH of the solutions were also measured. Nonparametric tests were used to determine statistical differences (P <.05). Results The presence of dentin powder resulted in a reduction of the free available chlorine and pH in all the irrigating solutions; 1% NaOCl lost its antimicrobial activity completely in the presence of dentin powder. The antimicrobial activity was significantly reduced in the 2.5% NaOCl and 1% NaOCl/HEBP groups, and it was not affected in the 2.5% NaOCl/HEBP group. Conclusions The presence of dentin powder significantly decreased the available chlorine and antimicrobial activity of 1% NaOCl, 2.5% NaOCl, and 1% NaOCl/HEBP irrigating solutions. The antimicrobial activity of 2.5% NaOCl/HEBP was not affected by the dentin powder after a 3-minute contact time against E. faecalis biofilms.

Original languageEnglish (US)
Pages (from-to)771-775
Number of pages5
JournalJournal of Endodontics
Issue number5
StatePublished - May 1 2016
Externally publishedYes

Bibliographical note

Funding Information:
Supported by the Research Group CTS-167 (Junta de Andalucía, Spain).


  • Antimicrobial activity
  • dentin debris
  • etidronic acid
  • root canal irrigants
  • sodium hypochlorite


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