Effects of colchicine on sulfated glycosaminoglycan production and cell detachment in pre-capillary pulmonary endothelial cells

C. Victoria Thomas; James L. Sackrison; Una S. Ryan; Sharon D Luikart

doi:10.1016/0040-8166(87)90069-3

Effects of colchicine on sulfated glycosaminoglycan production and cell detachment in pre-capillary pulmonary endothelial cells

C. Victoria Thomas
, James L. Sackrison
, Una S. Ryan
, Sharon D Luikart

Medicine - Veteran's Administration Medical Center

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The effects of colchicine on the morphology, substrate adhesiveness, and production of glycosaminoglycan (GAG) macromolecules by cultured pre-capillary pulmonary endothelial cell were studied. Colchicine-treated cells demonstrated altered morphology and decreased substrate adhesiveness compared to untreated cells. In addition, [³⁵S]sulfate incorporation into glycosaminoglycans was decreased 33% after treatment with colchicine. Spectrophotometric measurement of total cellular GAG revealed a similar GAG reduction in colchicine-treated cells. The composition of [³⁵S]sulfate radiolabelled GAG was similar in cultures with and without colchicine, consisting of approximately 56% chondroitin sulfate and the remainder heparin/heparan sulfate. The results indicate that colchicine influences the biological behavior of pre-capillary endothelial cells, in part by altering the amount of glycosaminoglycan molecules produced.

Original language	English (US)
Pages (from-to)	617-624
Number of pages	8
Journal	Tissue and Cell
Volume	19
Issue number	5
DOIs	https://doi.org/10.1016/0040-8166(87)90069-3
State	Published - 1987

Bibliographical note

Funding Information:
Grant CA-38972, awardedb y the National Cancer Institute, DHHS; Grant CH-39973 from the American Cancer Society;t he Min-nesotaM edical Foundation; and the Masonic Memorial Hospital Fund, Inc. U. Ryan is the recipiento f Grants HL-21568 and HL-33064 from the National Institutes of Health and Grant 814 from the Council for Tobacco Research-U.S.A., Inc.

Funding Information:
This work was supported, in part, by PHS

Keywords

Endothelial cells
colchicine
glycosaminoglycans
microtubule-disrupting agents

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10.1016/0040-8166(87)90069-3

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@article{3393046d2b9e4ca9af5a897b509599f1,

title = "Effects of colchicine on sulfated glycosaminoglycan production and cell detachment in pre-capillary pulmonary endothelial cells",

abstract = "The effects of colchicine on the morphology, substrate adhesiveness, and production of glycosaminoglycan (GAG) macromolecules by cultured pre-capillary pulmonary endothelial cell were studied. Colchicine-treated cells demonstrated altered morphology and decreased substrate adhesiveness compared to untreated cells. In addition, [35S]sulfate incorporation into glycosaminoglycans was decreased 33\% after treatment with colchicine. Spectrophotometric measurement of total cellular GAG revealed a similar GAG reduction in colchicine-treated cells. The composition of [35S]sulfate radiolabelled GAG was similar in cultures with and without colchicine, consisting of approximately 56\% chondroitin sulfate and the remainder heparin/heparan sulfate. The results indicate that colchicine influences the biological behavior of pre-capillary endothelial cells, in part by altering the amount of glycosaminoglycan molecules produced.",

keywords = "Endothelial cells, colchicine, glycosaminoglycans, microtubule-disrupting agents",

author = "Thomas, \{C. Victoria\} and Sackrison, \{James L.\} and Ryan, \{Una S.\} and Luikart, \{Sharon D\}",

note = "Funding Information: Grant CA-38972, awardedb y the National Cancer Institute, DHHS; Grant CH-39973 from the American Cancer Society;t he Min-nesotaM edical Foundation; and the Masonic Memorial Hospital Fund, Inc. U. Ryan is the recipiento f Grants HL-21568 and HL-33064 from the National Institutes of Health and Grant 814 from the Council for Tobacco Research-U.S.A., Inc. Funding Information: This work was supported, in part, by PHS",

year = "1987",

doi = "10.1016/0040-8166(87)90069-3",

language = "English (US)",

volume = "19",

pages = "617--624",

journal = "Tissue and Cell",

issn = "0040-8166",

publisher = "Elsevier Ltd",

number = "5",

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TY - JOUR

T1 - Effects of colchicine on sulfated glycosaminoglycan production and cell detachment in pre-capillary pulmonary endothelial cells

AU - Thomas, C. Victoria

AU - Sackrison, James L.

AU - Ryan, Una S.

AU - Luikart, Sharon D

N1 - Funding Information: Grant CA-38972, awardedb y the National Cancer Institute, DHHS; Grant CH-39973 from the American Cancer Society;t he Min-nesotaM edical Foundation; and the Masonic Memorial Hospital Fund, Inc. U. Ryan is the recipiento f Grants HL-21568 and HL-33064 from the National Institutes of Health and Grant 814 from the Council for Tobacco Research-U.S.A., Inc. Funding Information: This work was supported, in part, by PHS

PY - 1987

Y1 - 1987

N2 - The effects of colchicine on the morphology, substrate adhesiveness, and production of glycosaminoglycan (GAG) macromolecules by cultured pre-capillary pulmonary endothelial cell were studied. Colchicine-treated cells demonstrated altered morphology and decreased substrate adhesiveness compared to untreated cells. In addition, [35S]sulfate incorporation into glycosaminoglycans was decreased 33% after treatment with colchicine. Spectrophotometric measurement of total cellular GAG revealed a similar GAG reduction in colchicine-treated cells. The composition of [35S]sulfate radiolabelled GAG was similar in cultures with and without colchicine, consisting of approximately 56% chondroitin sulfate and the remainder heparin/heparan sulfate. The results indicate that colchicine influences the biological behavior of pre-capillary endothelial cells, in part by altering the amount of glycosaminoglycan molecules produced.

AB - The effects of colchicine on the morphology, substrate adhesiveness, and production of glycosaminoglycan (GAG) macromolecules by cultured pre-capillary pulmonary endothelial cell were studied. Colchicine-treated cells demonstrated altered morphology and decreased substrate adhesiveness compared to untreated cells. In addition, [35S]sulfate incorporation into glycosaminoglycans was decreased 33% after treatment with colchicine. Spectrophotometric measurement of total cellular GAG revealed a similar GAG reduction in colchicine-treated cells. The composition of [35S]sulfate radiolabelled GAG was similar in cultures with and without colchicine, consisting of approximately 56% chondroitin sulfate and the remainder heparin/heparan sulfate. The results indicate that colchicine influences the biological behavior of pre-capillary endothelial cells, in part by altering the amount of glycosaminoglycan molecules produced.

KW - Endothelial cells

KW - colchicine

KW - glycosaminoglycans

KW - microtubule-disrupting agents

UR - https://www.scopus.com/pages/publications/0023492994

UR - https://www.scopus.com/pages/publications/0023492994#tab=citedBy

U2 - 10.1016/0040-8166(87)90069-3

DO - 10.1016/0040-8166(87)90069-3

M3 - Article

C2 - 3424336

AN - SCOPUS:0023492994

SN - 0040-8166

VL - 19

SP - 617

EP - 624

JO - Tissue and Cell

JF - Tissue and Cell

IS - 5

ER -

Effects of colchicine on sulfated glycosaminoglycan production and cell detachment in pre-capillary pulmonary endothelial cells

Abstract

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