Effects of colchicine on sulfated glycosaminoglycan production and cell detachment in pre-capillary pulmonary endothelial cells

C. Victoria Thomas, James L. Sackrison, Una S. Ryan, Sharon D Luikart

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

The effects of colchicine on the morphology, substrate adhesiveness, and production of glycosaminoglycan (GAG) macromolecules by cultured pre-capillary pulmonary endothelial cell were studied. Colchicine-treated cells demonstrated altered morphology and decreased substrate adhesiveness compared to untreated cells. In addition, [35S]sulfate incorporation into glycosaminoglycans was decreased 33% after treatment with colchicine. Spectrophotometric measurement of total cellular GAG revealed a similar GAG reduction in colchicine-treated cells. The composition of [35S]sulfate radiolabelled GAG was similar in cultures with and without colchicine, consisting of approximately 56% chondroitin sulfate and the remainder heparin/heparan sulfate. The results indicate that colchicine influences the biological behavior of pre-capillary endothelial cells, in part by altering the amount of glycosaminoglycan molecules produced.

Original languageEnglish (US)
Pages (from-to)617-624
Number of pages8
JournalTissue and Cell
Volume19
Issue number5
DOIs
StatePublished - 1987

Keywords

  • Endothelial cells
  • colchicine
  • glycosaminoglycans
  • microtubule-disrupting agents

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