Abstract
The effects of colchicine on the morphology, substrate adhesiveness, and production of glycosaminoglycan (GAG) macromolecules by cultured pre-capillary pulmonary endothelial cell were studied. Colchicine-treated cells demonstrated altered morphology and decreased substrate adhesiveness compared to untreated cells. In addition, [35S]sulfate incorporation into glycosaminoglycans was decreased 33% after treatment with colchicine. Spectrophotometric measurement of total cellular GAG revealed a similar GAG reduction in colchicine-treated cells. The composition of [35S]sulfate radiolabelled GAG was similar in cultures with and without colchicine, consisting of approximately 56% chondroitin sulfate and the remainder heparin/heparan sulfate. The results indicate that colchicine influences the biological behavior of pre-capillary endothelial cells, in part by altering the amount of glycosaminoglycan molecules produced.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 617-624 |
| Number of pages | 8 |
| Journal | Tissue and Cell |
| Volume | 19 |
| Issue number | 5 |
| DOIs | |
| State | Published - 1987 |
Bibliographical note
Funding Information:Grant CA-38972, awardedb y the National Cancer Institute, DHHS; Grant CH-39973 from the American Cancer Society;t he Min-nesotaM edical Foundation; and the Masonic Memorial Hospital Fund, Inc. U. Ryan is the recipiento f Grants HL-21568 and HL-33064 from the National Institutes of Health and Grant 814 from the Council for Tobacco Research-U.S.A., Inc.
Funding Information:
This work was supported, in part, by PHS
Keywords
- Endothelial cells
- colchicine
- glycosaminoglycans
- microtubule-disrupting agents